摘要
利用抗坏血酸(VC)还原银氨溶液生成具有强烈等离子体共振散射特性的银纳米粒子,导致体系共振光散射信号的增强,散射最大峰位于544nm。在最佳条件下,体系的共振散射强度与抗坏血酸浓度在0.2～7.0μmol/L范围内呈线性关系,相关系数为0.9988,检出限为8.1nmol/L。抗坏血酸的氧化产物能与银纳米粒子相互作用使之分散均匀且保持稳定,因此该体系不需要添加稳定剂或分散剂。以此建立了用银纳米粒子为探针的测定抗坏血酸的高灵敏、简捷的共振散射新方法。同时讨论了最佳反应条件和其它影响因素。该方法用于VC片及饮料中抗坏血酸的测定,加标回收率在94.5%～97.0%之间。结果表明,方法具有操作简单,灵敏度高和回收率较好等优点。
A resonance light scattering detection method of ascorbic acid is reported based on the formation of silver nanoparticles（NPs）.Ascorbic acid（vitamin C,VC） could reduce Ag（NH3） 2＋ to form the silver nanoparticles,resulting in strong plasmon resonance light scattering（PRLS） signals characterized at 544 nm,which could be measured using a common spectrofluorometer.It is found that the PRLS intensities were proportional to the ascorbic acid concentrations in the range of 0.2～7.0 μmol/L with R2=0.9988 and detection limit（3σ/k） of 8.1×10-9 mol/L.The presence of a physically adsorbed layer of the product of the redox reaction on the surface of colloidal silver could prevent irreversible aggregation of silver NPs.The conditions of the reaction and the interference of the common ions are investigated.For the determination of ascorbic acid in VC tablets and beverage samples.The recovery was within the range of 94.5% ～ 97.0%.Results suggested that the method possessed easy of implementation,high sensitivity,and better recovery,and so on.
出处
《应用化学》
CAS
CSCD
北大核心
2010年第12期1474-1477,共4页
Chinese Journal of Applied Chemistry
关键词
检测
银纳米
共振散射
抗坏血酸
detection
silver nanoparticles
resonance light scattering
ascorbic acid
