摘要
建立高效液相色谱法同时测定谷瑞德胶囊中谷胱甘肽及维生素C含量的方法。色谱柱为InertSustain C_(18)柱(250 mm×4.6 mm,5μm);以0.025 mol/L磷酸二氢钠溶液(用磷酸调pH 3.0)为流动相;检测波长为220 nm。结果表明,谷胱甘肽与维生素C的质量浓度分别在65.592～1 311.84,20.632～412.64μg/mL范围内与色谱峰面积呈良好的线性关系,线性相关系数均为1,检出限分别为16,177 ng/mL。谷胱甘肽与维生素C的加标回收率分别为97.1%～99.1%,98.2%～100.6%,测定结果的相对标准偏差分别为0.66%,0.87%(n=6)。该方法灵敏度高、重现性好,稳定可控,可为谷瑞德胶囊质量标准的建立提供技术支持。
The method for simultaneous determination of glutathione and vitamin C in guruide granules by high performance liquid chromatography was established. Chromatographic column was InertSustain C18（250 mm×4.6 mm,5 μm）, the mobile phase was 0.025 mol/L sodium dihydrogen phosphate solution（adjust pH 3.0 with phosphoric acid）,the detection wavelength was 220 nm. The results showed that the mass concentration of glutathione and vitamin C had good linear relationship with the chromatographic peak area in the range of 65.592–1311.84, 20.632–412.64 μg/mL, respectively, the linear correlation coefficients both were 1. The detection limits of glutathione and vitamin C were 16 ng/mL and 177 ng/mL, respectively. The recovery was 97.1%–99.1%, 98.2%–100.6% for glutathione and vitamin C, respectively. The relative standard deviations of determination results were 0.66%, 0.87%（n=6） for glutathione and vitamin C, respectively. This method is stable and controllable with the high sensitivity and good reproducibility, which can provide technical support for the quality standard establishment of guruide granules.
作者
朱倩云
方健华
李成网
张洁
白娟
周秀红
ZHU Qianyun1, FANG Jianhua2, LI Chengwang1, ZHANG Jie1, BAI Juan1, ZHOU Xiuhong1(1. Anhui Academy of Medical Sciences, Hefei 230061, China;2. Anhui Zhengxing Biotechnology Co., Ltd., Xuancheng 245300, China)
出处
《化学分析计量》
CAS
2018年第4期94-97,共4页
Chemical Analysis And Meterage
关键词
高效液相色谱法
谷瑞德胶囊
谷胱甘肽
维生素C
high performance liquid chromatography
guruide granules
glutathione
vitamin C
