摘要
观察抗纤复方对大鼠传代培养肝贮脂细胞自分泌产生ＴＧＦβ１的影响。方法采用血清药理学方法处理贮脂细胞，以貂肺上皮细胞（Ｍｖｌｌｕ）生长抑制ＭＴＴ法检测培养上清中ＴＧＦβ１活性，并以小牛血清、正常鼠血清和秋水仙碱药物血清为对照。结果抗纤复方药物血清和秋水仙碱药物血清均能明显抑制贮脂细胞产生ＴＧＦβ１，而且抗纤复方在低药物血清浓度（５％、１０％）时作用更明显。经抗ＴＧＦβ１抗体中和实验验证了检测ＴＧＦβ１的特异性。结论抗纤复方能抑制贮脂细胞产生ＴＧＦβ１，阻断肝纤维化时的贮脂细胞自分泌放大过程，减少胶原等ＥＣＭ的合成。
Aims To observe the influence of kangxian compound recipe(KXCR, compound herb recipe for antifibrosis)on the autocrine of TGF of in rat subcultUred liver fat-storing cells(rsC). Methods The serum pharmacology method wasemployed to treat the FSC, and the caF β1 activity in the supernatant Of culture was measured by bank lung epithilial cell(MviIu)proliferation inhibition Mad assay. The cells treated by bovine serum, noneal rat semm and colchicine were taken as controlgroups.Results Serum from either KXCR or colchicine group could remarkably inhibit the yield of TGFβ1 in the FSC, andserum fain the former group whibited a more significant effect when at lower concentrations(5%, 10% ). The specifity in testing TGFβ1 was affirmed by anti-TGFβ1 antibody neutralization experiment. COncluSions KXCR may inhibit the Production ofTGFβ1 in FSC, thus, block the FSC autocrine magnification pcness and reduce the synthesis of ECM such as collegen, etc during liver fibrosis.
出处
《胃肠病学和肝病学杂志》
CAS
1999年第1期16-17,共2页
Chinese Journal of Gastroenterology and Hepatology
