摘要
建立了纳米颗粒探针检测核糖体失活蛋白的方法。以聚苯乙烯纳米颗粒为载体并进行表面修饰,在其表面固定特异性蓖麻毒单抗并作为探针,用于蓖麻毒蛋白的检测。采用场流分离技术对纳米颗粒探针进行准确表征,并利用扫描电镜比较聚苯乙烯纳米颗粒的形貌变化。结果表明:通过扫描电镜图片能够观察到聚苯乙烯纳米颗粒表面结合的蛋白,此纳米颗粒探针能够与蓖麻毒蛋白等核糖体失活蛋白的特异性结合,应用于目标蛋白的检测。
A method with potential application in ribosome-inactivating proteins determination in real food matrix or human biofluids was described by utilizing nanoparticles as a carrier, based on specific antibody as capture probes immobilized on particulate substrates in the nm-range. Anti-ricin antibody modified by s-（2-pyridyldithio） propionic acid N-hydroxysuccinimide ester （SPDP） was attached to polystyrene nanoparticles surface via end-group-activated poly（ethylene glycol） block-poly（propylene glycol）-block-poly（ethylene glycol）（F108-PDS）, which can be used to determine ricin protein. Field flow fractionation approach was used to characterize the nanoparticle probe, the particle morphology was also observed by scanning electron microscope. The results indicated that the protein attached on the probe surface can be found from the SEM image and the prepared nano probe can be used to determine target ricin protein specifically.
出处
《分析化学》
SCIE
EI
CAS
CSCD
北大核心
2010年第5期627-631,共5页
Chinese Journal of Analytical Chemistry
基金
中国科技部创新方法工作专项(No.2008IM021900)资助
关键词
纳米颗粒探针
蓖麻毒素
核糖体失活蛋白
场流分离
扫描电镜
聚苯乙烯
Nanoparticle probe
Ricin
Ribosome-inactivating protein
Field flow fractionation
Scanning electron microscope
Polystyrene latex sphere
