摘要
利用纳米金对石英晶体微天平（QCM）的表面修饰和质量扩增效应，建立了一种压电免疫传感器检测蓖麻毒素的新方法。首先在石英晶体的金电极上依次自组装1，6-己二硫醇和纳米金进行表面修饰，然后通过蛋白A定向固定蓖麻毒素多抗来制备敏感膜。利用纳米金的质量扩增效应设计了一种“毒素-单抗-蛋白A-纳米金”复合物，成功实现了对蓖麻毒素的检测，提高了传感器灵敏度和特异性。该传感器对蓖麻毒素响应的线性范围为0．50—10mg／L，回归方程为△F=45．81 Cricin＋48．48（R=0．9986，N=10，P〈0．01）；检测灵敏度为45．81Hz／（mg／L）。
A novel approach for ricin detection by piezoelectric immunosensor has been developed, which is based on the surface modification on QCM （Quartz Crystal Microbalance ） by nanometer-sized gold （nanogold） particles and the mass multiplied effect of these particles. The Au electrode of the piezoelectric quartz crystal was firstly modified with 1,6-hexandithiol （HDT） self-assembled monolayer （SAM） to deposit nanogold particles and protein A （PA） , on which ricin polyclonalantibodies （PcAb）were successfully immobilized on PA layer through Fc domain. Depending on the mass multiplied effect of nano-gold particles, the ＂ricinmonoclonal antibody（McAb）-PA-nano-gold＂ immunocomplex was designed to enhance the response signals of the immunosensor, which improved the sensitivity and selectivity of the sensor. The detecting linear range of the piezoelectric immunosensor for ricin is 0.5 - 10 mg/L. The linear regression equation was △F = 45.81 Cricin ＋ 48.48 （R = 0. 9986, N = 10, P 〈 0.01 ） with the sensitivity of 45.81 Hz/（ rag/L）.
出处
《分析化学》
SCIE
EI
CAS
CSCD
北大核心
2006年第12期1779-1782,共4页
Chinese Journal of Analytical Chemistry
基金
总装预研课题资助项目(No.40407020204)
关键词
压电免疫传感器
纳米金
表面修饰
质量扩增效应
蓖麻毒素
Piezoelectric immunosensor, nano-gold, surface modification, mass multiplied effect, ricin
