摘要
目的研究高血压病患者过氧化物酶体增殖物激活受体γ2基因(PPARγ2)Pro12Ala多态性与认知功能之间的关系。方法纳入2008年1月至2009年2月在四川大学华西医院心内科就诊的四川地区汉族人群中原发性高血压患者502例,收集一般资料,采用国际通用的简易智力状况量表(MMSE)测验认知功能,按认知评分分为认知功能正常组(n=376)和认知功能障碍组(n=126),用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)技术测定PPARγ2基因Pro12Ala多态性。同时测定胆固醇、甘油三酯、空腹血糖、胰岛素及餐后2 h血糖、胰岛素水平。结果 502例原发性高血压患者等位基因型及基因频率的分布结果显示,PP基因型88.6%,PA基因型11.4%,AA基因型0%,P等位基因频率94.3%,A等位基因频率5.7%。其中认知功能正常组376例,PP和PA基因型分别为328例(87.2%)和48例(12.8%);认知功能障碍组126例,PP和PA基因型分别为126例(92.9%)和9例(7.1%)。两组基因型及等位基因频率分布显示差异均无统计学意义(P均>0.05),进一步按照性别和年龄分组后,认知功能正常组与认知功能障碍组之间基因型间差异无统计学意义。Logistic回归分析也未发现PPARγ2基因Pro12Ala多态性与高血压病认知功能相关〔PP基因型:OR=1;PA基因型:OR=0.511,95%可信区间(0.226,1.153),P=0.106〕。结论 Pro12Ala基因多态性与高血压病患者认知功能障碍无明显相关性。
Objective To study the relationship between PPARγ2 Pro12Ala polymorphism and cognitive function in patients with primary hypertension. Methods This study enrolled 502 hypertensive patients of Chinese Han population from Jan 2008 to Feb 2009 in West China Hospital of Sichuan University. We collected the general data and applied the mini mental state examination （MMSE） to test the cognitive function and computed score. Total cholesterol （TC）, triglyeride （TG）, fasting plasma glucose （FPG） and postprandial blood sugar （PPBS）, fasting insulin （FINS） and postprandial plasma insulin （PINS） were measured. PCR-RELP method was used to analysis the PPARγ2 Pro12Ala gene polymorphism. Results Pro12Pro genotype was present in 88. 6% of the patients and Pro12Ala genotype was present in 11.4% of the population. Allele frequencies were 94.3% for Pro allele and 5. 7% for Ala allele. In cognitive normal group, the frequencies of PP and PA genotype were 328 （87.2%） and 48 （12.8%）, while the frequencies of PP and PA genotypes in the cognitive dysfunction group were 126（92.9%）, 9 （7.1%） respectively. Analyzed by χ^2 test, both the genotype frequency and the allele frequency of PPARγ2 Pro12Ala polymorphism did not display statistical variability between the cognitive normal group and the cognitive dysfunction group, even eliminating the influence of age and sexuality. Conclusion Pro12Ala polymorphism in PPARγ2 with primary hypertension may not associate with cognitive impairment.
出处
《四川大学学报（医学版）》
CAS
CSCD
北大核心
2010年第6期1034-1038,共5页
Journal of Sichuan University(Medical Sciences)
