摘要
目的:研究葛根素对培养SD乳鼠心肌细胞缺氧/复氧(A/R)损伤的拮抗作用。方法:葛根素(50和100mg/L)预处理24h后作用于乳鼠心肌细胞A/R损伤模型,运用MTT法测定细胞存活率,比色法测定细胞培养液中LDH和细胞内SOD活性及MDA含量。运用流式细胞术Annexin V-PI双染法测定细胞凋亡变化。结果:与A/R模型组比较,葛根素明显提高细胞存活率,增加胞内SOD活性,降低培养液中LDH释放量和胞内MDA含量;同时降低细胞的凋亡率。结论:葛根素对A/R损伤心肌细胞具有保护作用,其机制可能与清除自由基的产生有关。
Objective：To investigate the protective effects of puerarin on cultured myocardiocytes in anoxia/reoxygenation injury.Methods：The cultured cardiocytes were pretreated with puerarin（50 and 100mg/L）.Cell viability,the activities of LDH SOD and the contents of MDA were determined respectively.The apoptosis ratio of myocardiocytes was determined by flow cytometry.Results：Puerarin could significantly improve myocardial cell viability in anoxia/reoxygenation injury,increase the activity of SOD,inhibit the release of LDH in supernatant,and decrease the contents of MDA and apoptosis rate of myocardiocytes.Conclusion：Puerarin has protective effect on myocardiocytes with anoxia/reoxygenation injury.
出处
《武汉大学学报（医学版）》
CAS
北大核心
2010年第6期763-765,共3页
Medical Journal of Wuhan University
