摘要
目的观察大蒜多糖（GP）对阿霉素（ADR）中毒心肌细胞的保护作用并探讨其机制。方法采用0～2d SD乳鼠心肌细胞做原代培养，复制ADR损伤心肌细胞模型，测定上清液及细胞中多项生化指标，并运用MTT比色法、流式细胞仪检测凋亡细胞。结果ADR（终浓度为1mg·L^-1）可致上清液肌酸激酶（CK）、乳酸脱氢酶（LDH）、谷草转氨酶（GOT）释放量增加（P〈0．01），同时细胞超氧化物歧化酶（SOD）活力下降而丙二醛（MDA）含量升高（P〈0．01），GP（25～100mg·L^-1）可呈浓度依赖性地降低CK、LDH、GOT活力，增加细胞SOD活力和降低MDA含量，尤其以GP大剂量组作用明显（P〈0．05或P〈0．01）。流式细胞仪检测GP能降低心肌细胞凋亡率，证实了GP的保护作用。结论GP能够拮抗ADR引起的自由基脂质过氧化，从而保护心肌细胞。
Aim To observe the protective effects and its mechanisms of Garlic Polysaccharide （GP） on toxic cardiac myocyte induced by adriamycin （ADR）. Methods Primary culture neonatal SD rat cardiac myocyte and ADR injury model were established. The activities of several superior fluid and cells enzymes were measured. Using MTT assay and flow cytometry, the apoptotic cardiac myocyte was shown. Results ADR increased the superior fluid creatine kinase （CK） , lactate dehydrogenase（ LDH ） , glutamic oxaloacetic transaminase （GOT）, and augmented myocardial malondialdehyde （MDA） content, while decreased the superoxide dismutase （ SOD ） activities （ P 〈 0. 01 ）. GP concentration-dependently caused a significant decrease of the activities of CK, LDH, GOT. It also increased SOD activities and depressed MDA content in cells. MTT assay and flow cytometry also showed GP could reduce cell apoptotic. Conclusion GP could protect cardiac myocyte through scavenging free radicals and inhibiting lipid peroxidation.
出处
《中国药理学通报》
CAS
CSCD
北大核心
2005年第9期1104-1107,共4页
Chinese Pharmacological Bulletin
关键词
大蒜多糖
阿霉素
心肌细胞
流式细胞仪
garlic polysaccharide
adriamycin
cardiac myocyte
flow cytometry
