摘要
目的:探讨胸腺嘧啶核苷(TdR)双阻断法对人胃癌细胞SGC-7901细胞周期的影响。方法:取对数生长期的SGC-7901细胞,加入2mmol/L、4mmol/L和8mmol/L TdR孵育15h,去除TdR孵育10h,再次加入不同浓度的TdR孵育15h后收集各组细胞,流式细胞术分析细胞周期各时相细胞百分比。经不同浓度TdR双阻断法诱导SGC-7901细胞后,再去除TdR孵育12h,收集各组细胞分析细胞周期各时相细胞百分比。结果:SGC-7901细胞经2mmol/L、4mmol/L和8mmol/L TdR双阻断法诱导,收获G0/G1期的细胞数分别为77.3%、77.5%和77.0%。再次去除TdR培养12h后,各期细胞百分比又可恢复正常范围。结论:2mmol/L TdR双阻断法诱导SGC-7901细胞即可在短时间内获得大量的G0/G1期细胞,是一种理想的获得大量处于"基态"的细胞的方法。
AIM： To investigate the effects of double thymidine deoxyribonueleoside （TdR） blocking on the cell cycle of human gastric cancer SGC-7901 cells. METHODS： SC, C- 7901 cells in the logarithm period were selected in the study and treated with TdR at concentration of 2 mmol/L, 4 mmol/L or 8 mmol/L for 15 h as the first blocking. After incubation in TdR -free medium for 10 h, the cells were treated with TdR at same concentrations again for another 15 h as the second blocking. The blocked cells were released by washing in fresh medium twice and incubation in TdR ,-free medium containing 10% fetal bovine serum for 12 h. The cells were collected and the cell cycle was detected by flow cy- tometry. RF^ULTS： By double TdR （2 mmoL/L, 4 mmol/L and 8 mmol/L） blocking to synchronize the cell cycle, the cells in G0/G1 phase accounted for 77. 3%, 77.5% and 77.0%, respectively. After further incubation for 12 h in TdR - free medium, the proportion of the cells in each phase of the cell cycles returned to normal range. CONCLUSION： The method of double TdR blocking is an ideal access in short term to acquire a large number of cells in G0/G, phase.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2010年第11期2097-2100,共4页
Chinese Journal of Pathophysiology
基金
国家自然科学基金资助项目(No.30973811)
