摘要
目的:探讨h FAM92A1在人宫颈癌He La细胞不同周期时相的表达差异。方法:常规培养Hela细胞,应用血清饥饿法将He La细胞同步化于G1期,胸腺嘧啶核苷双阻断法同步化于S、G2期,秋水仙素阻断法同步化于M期,应用流式细胞术检测同步化效率,应用RT-PCR方法检测h FAM92A1在人宫颈癌He La细胞不同周期时相的表达水平。结果:流式细胞仪检测细胞同步化效率G1期为77.5%、S期为85.5%、G2期为61.9%、M期为44%;FAM92A1基因在各期均有表达,但存在差异,以S期表达最高。结论:FAM92A1基因在He La细胞不同周期时相的表达有较大差异。
Objective： To investigate the expression differences of h FAM92A1 in different cell cycle time phases of He La cells of human cervical carcinoma. Methods： He La cells were conventionally cultured and synchronized in G1 phase using serum starvation,synchronized in G2 and S phase using double thymidine blocking,synchronized in M phase using colchicine blocking,and synchronizing efficiency was tested by flow cytometry. RT-PCR method was adopted to detect the expression of h FAM92A1 in different cell cycle phases of He La cells. Results： Flow cytometry instrument detected cell synchronization efficiency as follows： G1 phase 77. 5%,S phase 85. 5%,G2 phase 61. 9%,M phase 44%. FAM92A1 genes were expressed in each period but with differences,the highest expression was in S phase. Conclusions： FAM92A1 gene of He La cell expression in different cycle phases exhibit notable differences.
出处
《贵阳医学院学报》
CAS
2015年第3期304-306,共3页
Journal of Guiyang Medical College
基金
湖北医药学院硕士启动金项目(No:2012ZRQDJ09)
关键词
基因
子宫颈
癌
细胞周期
genes
cervix uteri
carcinoma
cell cycle
