摘要
目的 研究大鼠肝实质细胞癌细胞 (HTC)的粘弹特性及其与胶原蛋白Ⅳ裱衬人工基底膜的粘附特性 ,分析其在细胞侵袭转移过程中的生物学意义。方法 同步化G1期 ( 69.2 0 %～72 .10 % )和S期 ( 97.18%～ 98.94% )HTC细胞以胸腺嘧啶核苷和秋水仙碱顺序阻断法及胸腺嘧啶核苷双阻断法分别获得 ,以微管吸吮技术对细胞的粘弹性及其与人工基底膜的粘附性进行测定。结果 HTC细胞具有高弹性系数 (K1、K2 )和低粘性系数 ( μ)的特征 ( 186.5 2± 75 .60 )N/m2 、( 2 2 4.42± 114 .5 2 )N /m2 、( 3.12± 2 .2 5 )M·S·m-2 ,G1期细胞尤为明显 ( 2 15 .2 8± 5 0 .2 4)N/m2 、( 181.92± 10 2 .90 )N/m2 、( 2 .87± 1.2 7)M·S·m-2 ,与胶原蛋白Ⅳ裱衬的人工基底膜的粘附力与胶原蛋白Ⅳ的浓度密切相关 ,G1期细胞 ( 2 75 .86± 2 32 .80 )× 10 -10 N具有比S期细胞( 161.16± 12 0 .40 )× 10 -10 N更高的粘附力和标准差 ,差异有显著性 (P <0 .0 5 )。结论 HTC细胞 ,尤其是其G1期细胞 ,更有可能在血液循环中存活并直接在微循环中滞留粘附 ,并穿透血管基底膜实现远端转移。
Objective To study the viscoelastic pro pe rties and the adhesive forces of hepatoma cells on artificial basement membrane (collagen Ⅳ coated).Methods The viscoelastic properties and adhesive forces o f hepatoma cells were investigated by micropipette aspiration technique. The syn chronous G_1 phase (69.20%-72.10%) and S phase (97.18% to 98.94%) cell s were obtained though thymine-2-desoxyriboside and cochicine sequential blockag e met hod and double thymine-2-desoxyriboside blockage method respectively.Results High K_1 (186.52±75.60)N/m^2,K_2 (224.42±11 4.52)N/m^2 and low μ(3.12±2.25) M·S·m^(-2) were the general characterist ics of hepatoma cells,especially for G_1 phase cells ( 215.28±50.24) N/m^2, (181.92±102.90) N/m^2,(2.87±1.27) M·S·m^(-2). The adhesive forces of he patoma cells on artificial basement membrane were in direct proportion to the co ncentration of collagen Ⅳ,and G_1 phase (275.86±232.80)×10^(-10)N hepato ma cells had higher adhesive forces than S phase (161.16±120.40)×10^(-10)N ce lls. Conclusion Hepatoma cells,especially for G_1 phase cells ,were more capable of surviving in blood circulation,and sequestration in micr ocirculation and more capable of adhering to and getting through basement membra ne,yielding remote metastasis.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2001年第6期536-537,共2页
Chinese Journal of Experimental Surgery
基金
国家自然科学基金资助项目 (3950 0 0 37)
