摘要
本实验从猪囊尾蚴虫体中提取 Ｒ Ｎ Ａ， 根据已发表的 Ｂ 抗原（ Ａｇ Ｂ） 核苷酸序列设计一对引物， 用 Ｒ Ｔ Ｐ Ｃ Ｒ 扩增出 Ａｇ Ｂ 全基因， 以ｐ Ｕ Ｃ１１９ 为载体克隆， 转化到大肠杆菌 Ｊ Ｍ８３ 中， 经分析鉴定所扩增片段为 Ａｇ Ｂ 基因。
The present paper describes the cloning of AgB cDNA of Cysticercus cellulosae First of all,total RNA was extracted from Cysticercus cellulosae Then THE AgB-encoding gene,a 2.6 Kb cDAN fragment was amplified by reverse transcription-polymerase chain reaction (RT-PCR) and cloned into pUC119 At last,the AgBencoding cDNA was confirmed by restriction analysis and sequencing.
出处
《中国预防兽医学报》
CAS
CSCD
1999年第4期308-310,共3页
Chinese Journal of Preventive Veterinary Medicine
