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猪囊尾蚴表面蛋白及其抗原性的初步测定分析 被引量:4

Primary Detection and Analysis of the Surface Protein from Cysticercus Cellulosae and Its Antigenicity
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摘要 本文初步分析了猪囊尾蚴囊壁表面蛋白的特性。根据囊尾蚴漂洗液PAGE和SDS-PAGE考马斯亮蓝染色带分别显示了9条及14条蛋白区带,其中大部分与宿主骨骼肌组织的可溶性蛋白有一致的迁移率,但其中第5、7、10条蛋白带为其独有。漂洗液与囊液兔高免血清IE可出现1条沉淀弧;用漂洗液蛋白抗原对35例囊虫病人血清做ELISA,阳性率为60%。此外,对囊虫表面宿主蛋白的可能作用,做了初步探讨。 This paper proved that there were some proteins in rinsed fluid of Cys-ticercus cellulosae. The proteins were separated and analysed by means of PAGE and SDS-PAGE. The patterns showed 9 bands and 14 bands stained by coomassic blue stain in PAGE and SDS-PAGE respectively. At Least 3 bands in rinsed fluid possessed of differentiating from host soluble protiens, ut the, greater part of them were same as host. Ont precipitation band agaist rabbit hyperimmune sera which were sensitized by cyst fluid of C. cell-ulosae was found in IE. The sera of 35 cases of cysticercosis were detected with antigen in rinsed fluid with positive rate 60%. Besides, the origination and function of the surface proteins of C. cellulosae were discussed.
出处 《中国人兽共患病杂志》 CSCD 北大核心 1989年第1期17-19,共3页 Chinese Journal of Zoonoses
关键词 猪囊尾蚴 表面蛋白 抗原 Cysticercus cellulosae, Surface protein, Antigenicity, Primary detection.
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  • 1杜军,伊藤洋一,李淑红,连建安.猪囊尾蚴可溶性蛋白的提取和分析[J].中国寄生虫学与寄生虫病杂志,1994,12(2):155-155. 被引量:4
  • 2郑宇,刘桂菊.间接血凝试验血滴纸片法诊断囊虫病的实验研究[J].哈尔滨医科大学学报,1995,29(1):15-17. 被引量:3
  • 3王俊霞,彭郁葱,姚玉霞,孙树汉.猪囊尾蚴特异性抗原编码cDNA的阶段特异性表达[J].中国寄生虫学与寄生虫病杂志,1996,14(2):89-93. 被引量:16
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