摘要
目的：建立血竭药材中有效成分5-羟基-7-甲氧基黄烷的HPLC含量测定方法。方法：Dikma Diamonsil C18色谱柱（4.6 mm×150 mm,5μm）,流动相为乙腈-水（60∶40）,检测波长210 nm,柱温30℃,流速1 mL.min-1。结果：5-羟基-7-甲氧基黄烷在0.01~0.10μg呈良好线性关系,r=0.999 9。平均回收率100.1%（n=6）。结论：该方法简便快速、结果准确,可用于检测血竭药材中有效成分5-羟基-7-甲氧基黄烷的含量。
Objective： To establish a method for the determination of 5-hydroxy-7-methoxyflavan in Daemonorops draco by HPLC.Method： Dikma Diamonsil C18 column（4.6 mm×150 mm,5 μm）was used.The mobile phase consisted of（60∶40）.The temperature of column was 30 ℃.The flow rate was 1.0 mL·min-1 and the detection wavelength was at 210 nm.Result： The calibration curve was in good linearity with in the range from 0.01 to 0.10 μg（r=0.999 9）.The average recovery was 100.1%（n=6）.Conclusion： The method is simple,rapid,as well as precise and reliable,and can be used for the determination of 5-hydroxy-7-methoxyflavan in D.draco.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2010年第23期3192-3193,共2页
China Journal of Chinese Materia Medica
基金
澳门特别行政区科学技术发展基金项目(046/2006/A)
