摘要
目的:检测PTEN、AKT2和CD147在胃癌组织中的表达,并探讨其相互关系。方法:应用免疫组织化学方法检测65例胃癌及癌旁组织中PTEN、AKT2和CD147的表达情况。结果:PTEN蛋白在胃癌中表达的阳性率(58.46%)明显低于相应正常组织(100%)(P<0.01),其表达水平与组织分化程度、浸润深度、淋巴结转移及TNM分期有关(P<0.05)。AKT2蛋白在胃癌组织中表达的阳性率(67.69%)明显高于相应正常组织(26.15%)(P<0.01),其表达与淋巴结转移有关(P<0.01),与浸润深度、分化程度和TNM分期无关(P>0.05)。CD147蛋白在胃癌组织中表达的阳性率(86.15%)明显高于相应正常组织(16.92%)(P<0.01),其表达水平与浸润深度、淋巴结转移及TNM分期有关(P<0.05),与分化程度无关(P>0.05)。胃癌组织中PTEN和AKT2、PTEN和CD147蛋白表达之间呈明显负相关(P<0.01)。结论:PTEN的低表达或失表达,可能与AKT2、CD147的异常激活有一定联系,从而对胃癌的发生、发展起重要作用。
Objective：To investigate the expression of PTEN,AKT2 and CD147 protein in gastric carcinoma tissues and relationship between their expression and clinical pathology parameters.Methods：Immunohistochemical assay was used to detect expressions of PTEN AKT2 and CD147 in 65 gastric cancerand adjacent tissues.Results：The positive rate of PTEN was significantly lower in gastric cance（r58.46%）than that in control tissues（100%,P〈0.01）,and expression level of PTEN was related to histological differentiation,depth of invasion,lymphnode metatasis and TNM stage （P〉0.05）.The positive rate of AKT2 was significantly higherin gastric cancer（67.69%）than that in control tissues （26.15%,P〈0.01）,and expression level of AKT2 was significantly correlated with lymphnode metatasis （P〈0.01）.No correlation was observed with histological differentiation,depth of invasion and TNM stage （P〉0.05）.The positive rate of CD147 was significantly higher in gastric cance（r86.15%）than that in normal tissues（16.92%）,and expression level of CD147 was related to depth of invasion,lymphnode metatasis and TNM stage（P〈0.05）;no correlation was found between the expression level of CD147 and histological differentiation （P〉0.05）.PTEN was correlated negatively with AKT2（P〈0.01）and CD147（P〈0.01）expression.Conclusion：The decrease or deletion of PTEN expression may be related to the abnormal activation of AKT2 and CD147 protein,and play an important role in the initiation and development of human gastric cancer.
出处
《中国现代普通外科进展》
CAS
2010年第9期681-684,共4页
Chinese Journal of Current Advances in General Surgery
