摘要
通过对柑桔球形合子胚不同发育期的分离培养、不同基本培养基及附加物、蔗糖浓度等因素对柑桔台子胚离体培养影响的研究，结果表明：授粉后50d是柑桔合子胚离体培养的最适时期；White基本培养基比MS基本培养基更适于柑桔球形合子胚离体培养。适当增加培养基的渗透压和添加水解乳蛋白，特别是柚子胚乳，可提高幼胚的诱导率，有利于柑桔球形胚的发育。比较适宜的蔗糖浓度为10％～15％，水解乳蛋白浓度为400mg／L。
The influence of separating stages of citrus zygotic embryos, basic media and supplements, concentrations of sucrose, etc. were studied on the in vitro culture of citrus globose zygotic embryos in this experiment. The results showed that the best stage to separate zygotic embryos was 50 days after pollination, and the White basic medium was more suitable than MS medium for culture of zygotic embryos. It is favourable for the development of globose zygotic embryos and raising of the induction rate of young embryos to increase the osmotic pressures of media, and supplement lactalbumin hydrolysate (LH) and especially pummelo endosperm. The suitable concentration of sucrose was 10%～15% and LH was 400 mg/L.
出处
《热带作物学报》
CSCD
1999年第2期27-30,共4页
Chinese Journal of Tropical Crops
基金
福建省自然科学基金
关键词
柑桔
球形合子胚
离体培养
citrus globose zygotic embryo in vitro culture
