摘要
用SSR标记分析了金柑属(Fortunella)及柑橘属(Citrus)植物的亲缘关系。选取的22对柑橘属SSR引物中,有14对能在所有材料中扩增出带纹,表明柑橘属SSR引物在金柑属植物上具有一定的通用性。在18个材料中扩增得到275个位点,平均每对引物扩增出19.7个位点。用NTSYS-PC软件进行分析,结果表明,金柑属植物与宽皮柑橘的亲缘关系很近;SSR聚类结果支持长寿金柑种的地位;支持宁波金弹、融安金弹、蓝山金柑均为金弹的栽培种的结论。为金柑属种质的保存、育种和分类提供了分子水平上的依据。
Phylogenetic relationships among 18 accessions belonging to Fortunella and Citrus were investigated by using SSR markers, lg SSR primers were selected from 22 Citrus SSR markers. These primers generated 275 polymorphic alleles in 18 accessions , with an average of 19.7 alleles per primer. Cluster analysis via NTSYS-PC software showed that Citrus was close to Fortunella. The result of SSR analysis supported that the species status of Changshou kumquat;the Ningbo kumquat, Rong＇an kumquat and Lanshan kumquat are the same species of Fortunella; some of the Citrus SSR markers can be used in Fortunella. The result can be used for preservation, breeding and classification in Fortunella.
出处
《果树学报》
CAS
CSCD
北大核心
2006年第3期335-338,共4页
Journal of Fruit Science
基金
重庆市自然科学基金资助项目(编号:8573)。
关键词
金柑属
SSR
亲缘关系
Fortunella
SSR
Phylogenetic relationship
