摘要
在固体组织培养研究的基础上，改进培养基组成成分进行液体静置培养。本文从水、糖、琼脂对组培苗生长、增殖及培养成本三个方面进行研究。作者认为马铃薯组培苗块繁阶段培养基可以简化为液培。此法同时省去琼脂，以自来水代替蒸馏水，以食用白绵糖代替蔗糖，规模化应用效果良好，培养基成本降低了７２．９％。组培苗生长健壮，月增殖系数６．３～６．７，结薯正常。
On the basis of the study of solid culture medium,a modified liquid medium was suggested to be used for the micropropagation of potato plantlets in vitro. In this medium,the agar was omitted,sucrose substituted by sugar and distilled water by tap water.The experiment indicated that the plantlests cultured in this static liquid medium were vigorous and the coefficient of propagation could be 6367 per month.More importantly,the medium cost could be reduced to 729% compared with the solid medium.
出处
《马铃薯杂志》
1999年第2期75-78,共4页
Chinese Potato Journal
关键词
马铃薯
微繁
液体静置培养
potato,micropropagation,liquid medium
