摘要
建立双歧杆菌快速检测方法间接ＥＬＩＳＡ法．应用双歧杆菌细胞壁作为抗原所获得的抗血清，能够检测出双歧杆菌的最低浓度范围在１０５ｍＬ左右．在检测过程中无需纯化抗原．所建立的方法具有较强的特异性。
The indirect ELISA testing method for rapidly detecting Bifidobacterium bifidum has been developed. Mouse polyclonal antibodies against the cell wall from Bifidobacterium bifidum are obtained. The minimum value of Bifidobacterium bifidum 's concentration which is capable of being determinated is about 105 mL. Using the method can effectively differ bifidobacterium bifidum from other species of microbes without the purification of the antibody,which is highly specific.
基金
江苏省应用基础研究项目
