摘要
目的探讨在Ｔ细胞活化信号传递中特异性识别和杀伤肝癌抗原的Ｔ细胞受体（ＴＣＲ）Ｖβ基因亚家族的表达特征，及与蛋白质酪氨酸激酶（ＰＴＫ）活性的关系．方法用单克隆抗体ＣＤ２８＋Ｂ７１（ＣＤ８０）共刺激正常人外周血淋巴细胞（ＰＢＬｓ）后作用于肝癌细胞（ＢＥＬ７４０２），用ＲＴＰＣＲ，Ｓｏｕｔｈｅｒｎ印迹、Ｗｅｓｔｅｒｎ印迹分析ＴＣＲＶβ基因表达特征及ＰＴＫ含量．结果ＴＣＲＶβ７基因在体外选择性扩增，ｄ４表达水平最高，为２４％，对照组为６％，相应地ＰＴＫ的含量也较高，为５８％，对照组为１１％．结论Ｖβ７基因特异性识别肝癌抗原。
AIM To study
the T cell receptor (TCR) and V β genes subfamilies expression characters in recognizing
and killing hepatoma antigen and its relationship to activity of protein tyrosine kinase (PTK).
METHODS After monoclonal antibody CD28+CD80(B7.1) co stimulated healthy human
peripheral blood lymphocytes (PBLs), and acted on hepatoma cell (BEL 7402), TCR V β7
gene expression characters and PTK activity were analyzed with PT PCR, Southern blot and
Western blot. RESULTS TCR V β7 gene was amplified selectively, the expression level
was the highest (24%), at day 4 in experimental group, but in the control group, 6%. The PTK
was 58% in experimental group, but in the control group, 11%. CONCLUSION V β7 gene is
special in recognizing hepatoma antigen, inducing the activation of PTK signal translation.
出处
《世界华人消化杂志》
CAS
1999年第4期288-290,共3页
World Chinese Journal of Digestology
基金
国家自然科学基金
广东省科教兴医"五个一"工程重点课题
关键词
肝肿瘤
T细胞受体
VΒ基因
基因表达
酪氨酸激酶
liver neoplasms
T cell receptor Vβ gene
gene expression
protein tyrosine
kinase
