摘要
目的分析IL-17+CD8+T淋巴细胞(Tc17)在肝细胞癌(HCC)患者肿瘤浸润淋巴细胞(TILs)中的表达,并初步探讨其意义。方法免疫组化技术检测HCC肿瘤组织及正常肝组织IL-17的表达;多色流式技术检测HCC患者肿瘤浸润淋巴细胞(TILs)、外周血单个核细胞(PBMCs)及健康者PBMCs中Tc17的频率,并分析TILs中Tc17的表型和功能;比较TNMⅠ、Ⅱ和Ⅲ期患者TILs中Tc17频率的差异。结果 HCC患者肿瘤组织IL-17表达阳性率显著高于正常肝组织(P<0.05)。HCC患者TILs中Tc17频率为(2.41±1.45)%,显著高于患者外周血(0.71±0.29)%和健康者外周血(0.51±0.37)%(P<0.05)。TILs中的Tc17细胞具有活化的效应记忆细胞表型(CD45RA-CD28+CD27-/+CCR7-CD69high),高表达炎性因子TNF和IFN-γ,几乎不表达颗粒酶B和穿孔素。TNM各期患者肝内Tc17细胞频率不具有显著性差异。结论 Tc17在HCC患者肝内TILs中表达上调,这些细胞具有活化的效应记忆细胞表型,几乎不表达颗粒酶B和穿孔素,但高表达炎性因子TNF和IFN-γ,可能在促进肿瘤的发生发展中发挥了重要作用。
In this study,we aimed to investigate the expression of IL-17＋CD8＋T lymphocytes（Tc17） in cancer tissues of patients with hepatocellular carcinoma（HCC） and its clinical usage.Immunohistochemisty staining was used to detect the expression of IL-17 in HCC and normal liver tissues,and flow cytometry was carried out to determine the distribution,phenotype and function of Tc17 cells in HCC patients.The expression diversities among different tumor stages were analyzed.We found that the positive rate of IL-17 expression was significantly higher in the HCC liver tissues than that in the normal liver tissues（P 0.05）;the frequency of Tc17 cells was significantly increased in tumor-infiltrating lymphocytes（TILs）（2.41±1.45）% compared with peripheral blood from patients（0.71± 0.29）% and healthy controls（0.51±0.37）%.Tc17 cells in TILs expressed an activated effector memory phenotype（CD45RA-CD28＋ CD27-/＋CCR7-CD69high） and expressed high levels of proinflammatory factors TNF and IFN-γ,but little granzyme B or perforin.Furthermore,we found no significant changes of Tc17 expression among different tumor stages.The results of this present work imply that Tc17 cells with activated effector memory phenotypic features are significantly accumulated in TILs of HCC patients.These cells secreted hardly cytotoxic molecules granzyme B or perforin,but large amounts of proinflammatory factors TNF and IFN-γ,through which they potentially contribute to tumorigenesis and tumor progression in HCC.
出处
《免疫学杂志》
CAS
CSCD
北大核心
2010年第11期967-972,共6页
Immunological Journal
基金
国家自然科学基金面上项目(30872344)
重庆市自然科学基金计划项目(CSTC2010BB5381)
重庆市卫生局医学科研计划面上项目(2010-2-436)
