摘要
选用玉米蛋白粉为原料,通过蛋白酶预处理再进行有机溶剂提取的方式制备叶黄素。与玉米蛋白粉中叶黄素的非酶解直接提取法相比,原料玉米蛋白粉采用最佳蛋白酶水解工艺预处理后(中性蛋白酶,酶用量为14000U/g玉米蛋白粉,液固比为20:1(mL/g),温度40℃,水解时间5h),不但使叶黄素的提取量由非酶解直接提取的165.3μg/g提高到340.5μg/g,而且浸提时间由5h缩短为20min。
After being pre-treated with protease,corn gluten meal received organic solvent extraction to obtain xanthophylls. Neutral protease was selected out of three commercially available proteases to pretreat corn gluten meal due to the highest extraction efficiency of xanthophylls,and methanol was found to be the best solvent for the extraction of xanthophylls among the ten solvents tested. The optimal conditions for neutral protease hydrolysis were determined to be：enzyme load,14000 U/g substrate,liquid/solid ratio,20：1; reaction temperature 40 ℃; and reaction duration,5 h. Compared with direct extraction without proteolytic pre-treatment,the optimized neutral protease hydrolysis resulted in an increase of extraction efficiency of xanthophylls from 165.3 to 340.5 μg/g and a reduction of extraction duration from 5 hour to 20 min.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2010年第20期52-57,共6页
Food Science
关键词
叶黄素
玉米蛋白粉
蛋白酶水解
提取
xanthophylls
corn gluten meal
proteolytic hydrolysis
extraction
