摘要
目的 将重组AdCTLA4Ig/Adα4β7诱导的耐受性树突细胞（DC）回输于卵清蛋白（OVA）致敏小鼠体内,观察其对食物过敏小鼠的治疗效果,为临床治疗儿童食物过敏提供新的思路.方法 建立BALB/c小鼠食物过敏模型.BALB/c雌鼠24只,分为3组：肠道激发4 h后回输耐受性DC为治疗组,回输生理盐水为阴性对照组和OVA过敏小鼠为阳性对照组.ELISA测定各组血清中OVA特异性IgE水平;HE染色观察空肠形态;免疫组化法检测小鼠小肠组织中白介素10（IL-10）;免疫荧光法检测转化生长因子β（TGF-β）表达水平.结果 ELISA法检测小鼠血清中OVA特异性IgE水平阳性对照组为（0.25±0.05）、阴性对照组为（0.14±0.04）、治疗组为（0.17±0.03）.治疗组较阴性对照组小鼠差异无统计学意义（P＞0.05）,而较阳性对照组过敏小鼠明显降低,差异有统计学意义（P＜0.05）.HE染色可见治疗组小肠绒毛中上皮细胞局灶性坏死、脱落,固有层炎症细胞浸润现象明显改善;IL-10表达增高.各组TGF-β表达无差异.结论 AdCTLA4Ig/Adα4β7修饰的耐受性DC对卵清蛋白过敏小鼠具有治疗作用,可以减轻肠道炎症反应,促进免疫耐受形成.
Objective To investigate the therapeutic effects of AdCTLA4 Ig/Adα4β7-dendritic cell （DC） in the ovalbulmin （OVA） allergic BALB/c mice. Method Twenty-four Female BALB/c mice aged 4-6 weeks fed on the ovalbulmin-free feed were randomly divided into 3 groups： （ 1 ） treatment group： the OVA sensitized mice were injected with tolerogenic AdCTLA4- Ig/Adα4β7-DC after being challenged with OVA. （2） negative control group： mice were sensitized and challenged with normal saline. （3） positive control group： mice were sensitized and challenged with OVA. The level of the OVA-specific IgE in serum was measured by ELISA. The jejunal samples were observed histologically after HE staining. The level of IL-10 and TGF-β in murine intestine was detected by immunohistochemical and immunofluorescent methods, respectively. Result Compared with the positive group, caudal vein injection with AdCTLA4Ig/Adα4β7-DC in treatment group reduced the level of the OVA-specific IgE in the serum remarkably, inhibited the inflammatory reactions of the intestine and increased the expression of the IL-10 in intestine significantly.There was no significant change in the expression of TGF-β in each group. Conclusion This study demonstrated that caudal vein injection with tolerogenic AdTLA4Ig/Adα4β7-DC may be of potential research value in the treatment of food allergy by inducing immune tolerance in vivo.
出处
《中华儿科杂志》
CAS
CSCD
北大核心
2010年第11期839-842,共4页
Chinese Journal of Pediatrics
基金
国家自然科学基金（30400408）
