摘要
目的 研究全反式维甲酸（all-trans retinoic acid,atRA）对卵清蛋白（ovalbumin,OVA）过敏小鼠的免疫治疗效果.方法 将40只OVA过敏小鼠按atRA干预剂量不同分为高剂量atRA组（100 mg/kg）、中剂量atRA组（50 mg/kg）、低剂量atRA组（20 mg/kg）以及对照组（无atRA）.观察不同剂量atRA干预12 d后小鼠体重、腹泻及肠道形态学变化;并采用酶联免疫吸附试验检测血清OVA-IgE、粪便总IgA及OVA-IgA含量;流式细胞仪检测肠系膜淋巴结中CD4＋ CD25＋ FoxP3＋T细胞占总CD4＋T细胞百分比.结果 与对照组相比,低剂量atRA组小鼠血清OVA-IgE（1.221±0.367比0.793±0.616）和粪便OVA-IgA（1.573±0.656比0.905±0.279）显著降低（t=3.140,P=0.006;t＝2.827,P=0.012）;小鼠空肠形态学基本正常;肠系膜淋巴结中CD4＋ CD25＋ FoxP3＋T细胞占总CD4＋T细胞百分比（10.641±1.218比10.936±0.954）差异无统计学意义（t=0.539,P=0.598）.而中、高剂量atRA两组小鼠血清OVA-IgE（1.109 ±0.319;0.938±0.281）、粪便总IgA（1.104±0.358;1.069 ±0.221）及肠系膜淋巴结中CD4＋ CD25＋ FoxP3＋T细胞占总CD4＋T细胞百分比（9.968±2.465;12.146 ±2.159）均与对照组间差异无统计学意义（P＞0.05）;同时中、高剂量atRA干预会造成小鼠体重下降及肠道黏膜修复异常.结论 低剂量atRA对OVA过敏小鼠可能有一定的免疫抑制作用;而中、高剂量atRA对OVA过敏小鼠无免疫治疗作用.
Objective The incidence of food allergy has increased in recent years and there is no effective way to treat it except strict dietary avoidance and rapid medical treatment in case of accidental exposure.Oral tolerance,as a new method,has shown great promise as an alternative approach to prevention and treatment for allergic disease.It was reported that all-trans retinoic acid （atRA） plays an important role in inducing oral tolerance in vitro.Our study aimed to investigate the immunological effect of different doses of atRA on ovalbumin （OVA） allergic BALB/c mice.Method BALB/c mice were sensitized by intraperitoneal injection with OVA to establish allergic animal model.According to the dose of atRA given,40 OVA allergic BALB/c mice were divided into 4 groups：the mice in high dose group were treated with 100 mg/kg atRA （atRA-H）,those in median dose group were treated with 50 mg/kg atRA （atRA-M）,those in low dose group were treated with 20 mg/kg atRA （atRA-L） and the mice in control group were given vehicle-soy oil only （CTR）.After 12 days of atRA intervention,weight was measured,the mice were checked for diarrhea,and intestinal histology was observed after hematoxylin and eosin staining.The level of OVA-IgE in serum,total IgA and OVA-IgA in feces were measured by ELISA.The percentage of CD4 ＋CD25 ＋ FoxP3 ＋ T cells in CD4 ＋ T cells in mesenteric lymph node was detected by flow cytometry.Result Compared with that of CTR group,the level of OVA-IgE in serum （1.221 ±0.367 vs.0.793 ±0.616） and OVA-IgA （1.573 ± 0.656 vs.0.905 ± 0.279） in feces decreased significantly （P =0.006 and 0.012,respectively） without weight and intestinal histology changes after low dose of atRA administration.However,there was no significant difference in the percentage of CD4 ＋ CD25 ＋ FoxP3 ＋ T cells in CD4 ＋ T cells in mesenteric lymph node （10.641 ± 1.218 vs.10.936 ± 0.954） between atRA-L and CTR group （P ＞ 0.05）.While in animals with high and median dose of atRA administration,no immunologic improvement was found,instead,there was weight loss and intestinal mucosal damage.Conclusion Low dose of atRA intervention seems to induce immune suppression in vivo resulting in positive effccts on OVA allergic mice.However,median and high dose atRA had no therapeutic effect on OVA allergic mice.
出处
《中华儿科杂志》
CAS
CSCD
北大核心
2014年第9期667-672,共6页
Chinese Journal of Pediatrics
基金
重庆市自然科学基金计划项目（CSTC,2011BB5135）
关键词
食物过敏
全反式维甲酸
治疗
Food allergy
All-trans retinoic acid
Therapy
