硝酸酯在小鼠急性实验性结肠炎中的治疗作用被引量：1

Therapeutic effect of nitrate on dextran sulfate sodium induced acute experimental colitis in mice

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摘要目的观察硝酸酯对小鼠急性实验性结肠炎的疗效。方法将40只BALB／c小鼠均分为模型组和治疗组，模型组予4％葡聚糖硫酸钠（DSS）溶液，治疗组予4VooDSS溶液和1．5g／L硝酸酯溶液，均连续饮用7d。对小鼠疾病活动指数（DAI）进行评分。取小鼠结肠组织进行苏木精-伊红染色和髓过氧化物酶（MPO）免疫组织化学染色并进行观察。分别用MPO和一氧化氮检测试剂盒检测结肠组织MPO和一氧化氮活性。统计学处理采用t检验。结果第6和第7天治疗组和模型组的DAI差异有统计学意义（t=5．12和6．72，P=0．012和0．008）。第7天时模型组组织学评分（2．5±0．5）高于治疗组（1．9±0．4），差异有统计学意义（F3．82，P〈0．01）。与模型组相比，治疗组小鼠结肠组织病理损伤明显减轻，中性粒细胞浸润减少。第7天时模型组MPO活性、NO2浓度、NO3浓度分别为（2．8±0．6）U／g、（10．4±4．3）mmol／g、（100．3±50．1）mmol／g，治疗组则分别为（1．5±0．3）U／g、（17．5±7．0）mmol／g、（190．7±85．3）mmol／g，差异均有统计学意义（t=11．23、3．81、4．50，P均〈0．01）。结论硝酸酯可减轻DSS诱导的小鼠急性实验性结肠炎。 Objective To investigate the effect of nitrate on acute experimental colitis in mice. Methods A total of 40 BALB/c mice were evenly divided into model group and treatment group. Model group were fed with 4% dextran sulfatesodium （DSS） solution and treatment group were given 40/00 DSS solution and nitrate （1.5 g/L） for seven days. The disease activity index （DAD of mice was scored. The colon tissue of mice was taken for hematoxylin-eosin staining and myeloperoxidase （MPO） immunohistochemical staining observation. The MPO and activity of nitric oxide in colon tissue were measured by MPO and nitric oxide detecting kit. The data were analyzed by t test. Results At the 6th day and 7th day, the difference of DAI between treatment group and model group was s^tatistically significant （t=5.12 and 6.72, P=0. 012 and 0. 008）. At the 7th day, the tissue score of model group （2.5 ± 0.5） was higher than that of treatment group （1.9 ± 0.4） and the difference was statistically significant （t=3.82, P〈0.01）. Compared with model group, the histopathological injury of colon tissue in treatment group mice significantly reduced and neutrophil infiltration also decreased. At the 7th day, the concentration of MPO, NO- and NO2- of model group was （2.8±0.6） U/g, （10.4± 4.3） mmol/g and （100.3±50.1） mmol/g respectively, treatment group was （1.5±0.3） U/g, （17.5± 7.0） mmol/g and （190.7± 85.3） mmol/g respectively. The differences were statistically significant（t=11.23, 3. 81 and 4. 50, all P〈0. 01）. Conclusion Nitrate can reduce DSS-induced acute experimental colitis in mice.

作者肖军华沈佳庆宋允娜郑萍

机构地区上海交通大学附属第一人民医院消化科

出处《中华消化杂志》 CAS CSCD 北大核心 2012年第12期830-833,共4页 Chinese Journal of Digestion

关键词结肠炎硝酸盐类一氧化氮过氧化物酶小鼠近交BALB C Colitis Nitrates Nitric oxide Peroxidase Mice, inbrecl BALB C

分类号 R574.62 [医药卫生—消化系统]

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