摘要
目的探讨两类树突状细胞mDC(CD11c+)和pDC(CD123+)在特应性皮炎(AD)中的变化及与疾病EASI评分的相关性。方法采用流式细胞仪检测20例特应性皮炎(AD)患者外周血中的mDC(CD11c+)和pDC(CD123+);计算mDC(CD11c+)/pDC(CD123+)比值;对AD患者进行EASI评分,应用Spearman相关性分析比较mDC(CD11c+)/pDC(CD123+)与EASI评分之间是否有相关性。以17名健康体检人员作为正常对照。结果 AD患者外周血中mDC(CD11c+)为(0.179±0.095)%,高于正常对照组(0.106±0.074)%(P<0.05);pDC(CD123+)为(0.112±0.069)%,高于正常对照组(0.063±0.049)%(P<0.05);AD患者与正常对照组mDC(CD11c+)/pDC(CD123+)差异无统计学意义(P>0.05);mDC(CD11c+)/pDC(CD123+)与EASI评分之间无明显相关性(P>0.05)。结论 AD患者外周血中两类树突状细胞mDC(CD11c+)和pDC(CD123+)均增高,提示在AD的发病中具有重要的意义。
Objective To observe the change of two types of dendritic cells, mDC（CDIIc ＋ ） and pDC（CI）123 ＋ ） in the peripheral blood of atopic dermatitis, and analysis il＇s relationship on EASI scores. Methods Flow eytometry was applied to assess mDC（ CD11c ＊ ） and pDC（ CD123 ＋ ） dendritic cell subsets in the peripheral blood of 20 patients with atopic dermatitis and 17 sex and age-matched human controls. The ml）C （CI）lle ＋ ）/pDC （ CD123 ＋ ） was eaeulated. Moreover, mDC （ CD11c＋ ）/pDC （ CD123 ＋ ） in patients with AD was campared to that in the controls, and the relationship between the mDC （CDIle ＋）/pDC （CD123 ＋ ） and the EAS1 scores was evaluted, by Spearman rank lesl. Results mDC（CD11c ＋ ） cells was（0. 179 ±0.095）% in patients with AD, this value was slightly, but significantly, higher than the（O. 106±0. 074）% in nnrmal subjects（P 〈 0.05）. Meanwhile, pDC（CD123＋） cells were（0. 112 ±0.069）% in patlenls with AD and（0. 063 ± 0. 049）% in normal subjects, respectively. The differences were aslu significant between the two groups（P 〈 0.05）. While there were no significant differences in the mDC（ CD11c ＋ ）/pDC（ CD123＋ ）, and the cowelation between mDC（CD11c＋ ）/pDC（CD123＋ ） and EASI scores in the two groups. Conclusion Two types of dendritic cells mDC（CD11c＋ ） and pDC（CD123＋） cells in peripheral blood were increased simultaneously, which suggested that the results may contribute to the the pathogenesis of AD.
出处
《中国皮肤性病学杂志》
CAS
北大核心
2010年第11期1011-1013,1016,共4页
The Chinese Journal of Dermatovenereology
基金
广州市医药卫生科技一般引导项目(2007-YB-139)
广东省医学科研基金(A2008581)
