摘要
利用SRAP(Sequence-related Amplified Polymorphism)分子标记技术构建茄子分子遗传连锁图,作图群体为255-4-4(Sola-num melongena L. sub sp. ovigerumSalis)与巴-3(S. melongena L. sub sp. melongena)杂交产生的118个F2单株。从88对引物中筛选多态性较好的33对引物组合进行群体检测,多态性频率为37.5%,共检测到40个多态性位点,平均每个引物组合产生1.21个多态性条带。对40个标记用Mapmanager构建连锁群,将24个SRAP标记位点进入4个连锁群,总长度为381.3 cM,标记位点间的平均距离为19.1 cM。
Molecular markers were valuable tools, widely used in genomic DNA analysis. Several molecular marker techniques had been in the development of DNA markers for map construction, which were useful in breeding, taxonomy, evolution and gene cloning. Based on the genetic analysis of SRAP marker for eggplant, the genetic linkage map was constructed with Mapmanager software by SRAP markers. DNA samples extracted from a F2 population of 255-4-4 （S. me/ongena L. subsp, ovigerum Salis） x Bai-3 （S. me/ongena L. subsp, me/ongena）. 33 of the 88 pairs of primers tested showed polymorphism and stability, ratio of polymorphism band was 37.5 %, 40 polymorphic loci were obtained from 33 pairs of primers and average was 1.21,24 of 40 loci were integrated into 4 linkage groups, which covered genome 381.3cM. The average distance between markers was 19.1 cM.
出处
《西南农业学报》
CSCD
北大核心
2010年第5期1591-1594,共4页
Southwest China Journal of Agricultural Sciences
基金
国家大宗蔬菜产业技术体系专项资金项目(NYCYTX-36)
四川省"十一五"农作物育种攻关项目(2006YZGG0601)
四川省育种工程财政专项项目(2006YZGC017)
