摘要
通过根癌农杆菌(Agrobacterium tumefaciens)介导法,以番茄叶片作外植体,将番茄(Sola-num lycopersicum L.)反义光敏色素A(PHYA)基因片段导入番茄。通过PCR扩增、Sourthernblot检测,证明反义光敏色素A的基因片段已整合到番茄基因组。在转基因番茄材料中,PHYA基因表达受到抑制,番茄红素的合成显著减少,果实没有表现出正常的红色果皮;转基因果实成熟过程中乙烯能够正常合成,与对照番茄之间没有显著差异。推测在调控番茄红素合成的模式中,光敏色素可能位于乙烯的下游位点起作用,二者共同调控番茄红素的合成。
Young leaves as explants, the tomato （Solanum lycopersicum L. ） was transformed with antisense genomic DNA of phytochrome A, which mediated by Agrobacterium tumefacien. It was shown by PCR analysis and Sourthem blotting detection that the fragment of PHYA was integrated into the tomato genome. Several results were observed from transgenic tomato friuts including inhibition of expression of PHYA, siginificant decrease in biosynthesis of lycopene and the abnormal color of the fruit skin. There were no siginificant differenecs between transgenic fruits and the control groups in the production of ethylene. Therefore, we presumed that phytochrome existing in the downstram of ethylene, which along with PHYA, played a role in the regulation of biosynthesis of lycopene. However, more studies on how both of them regulate the biosynthesis of lycopene are needed.
出处
《园艺学报》
CAS
CSCD
北大核心
2009年第5期679-684,共6页
Acta Horticulturae Sinica
基金
国家自然科学基金项目(30400304)
