摘要
以12个不同的茄子(Solanum melongena L.)高代自交系为试材,通过对AFLP反应体系中的几个关键因素进行优化,建立了适宜茄子的AFLP体系.结果表明,酶切基因组DNA适宜用量为100ng,酶切-连接适宜时间为10 h,预扩增产物适宜稀释倍数为30～50倍.
An AFLP (amplified fragment length polymorphism) analysis system was established with 12 eggplant (Solanum melongena L.) self-bred lines by optimizing some factors of the AFLP technological system. The results showed that the dosage of template DNA should be 100 ng, the time of digestion and ligation should be 10 hrs, and the products of preamplification should be diluted to 30~50 times.
出处
《西南农业大学学报（自然科学版）》
CSCD
北大核心
2005年第2期226-229,共4页
Journal of Southwest Agricultural University
基金
国家"863"资助项目(2002AA244011-1)
关键词
茄子
AFLP
影响因数
eggplant
AFLP system
influencing factor
