摘要
目的:建立蓝光诱导视网膜光感受器变性大鼠模型,评价其稳定性及可重复性。方法:选用成年雄性SD大鼠,予缝线开睑,1%阿托品扩瞳,经24h暗适应后,放入光照箱中接受24h强度为2 500Lux的宽谱蓝光照射。在光照后的1d、3d、7d、14d应用光镜、TUNEL染色、透射电镜观察实验大鼠视网膜组织结构的改变及细胞凋亡情况;应用闪光ERG评价视网膜功能。结果:光照后视网膜光感受器的内外节破坏,外核层排列紊乱,核质固缩,核层逐渐变薄,14d时减少达60.09%;光照后1d,外核层出现大量TUNEL(+)细胞,经透射电镜证实光感受器的死亡方式为凋亡;光照后14d,视网膜功能显著下降,0dB光照强度不能诱导出Photic-ERG波形。结论:2 500Lux蓝光照射能够诱导视网膜光感受器发生典型的以凋亡为特征的变性过程,具有较好的稳定性和可重复性。
Objective： To explore the stability and repeatability of the animal model of blue light-induced photoreceptor degeneration.Methods： Sprague-Dawley rats accepted dark-adaptation for 24 h prior to blue light exposure of 24 h at 2.5 Klux.At 1d,3d,7d and 14d after exposure to light,HE staining,TUNEL staining and transmission electron microscopy were used to observe the changes of retinal histology and apoptosis of photoreceptors.Dark-adapted flash ERG was used to evaluate the retinal funciton.Results： The inner and outer segment of photoreceptors were destroied,the outer nuclear layer（ONL） was disarranged and thinned with 60.09% reduction at 14d after exposure to light.There were a large amount of TUNEL（＋） apoptotic cells in the ONL at 1d which was confirmed by transmission electron microscopy.Both a-and b-wave amplitude were significantly reduced 14d after light exposure.Conclusions：Blue light exposure induced photoreceptor degeneration typical of apoptoses with excellent stability and repeatability.
出处
《中国临床医学》
2010年第5期618-622,共5页
Chinese Journal of Clinical Medicine
基金
国家自然科学基金青年科学基金项目(编号:30901641)
上海市青年科技启明星计划(编号:10QA1401200)
关键词
光
光感受器
凋亡
视网膜
变性
Light
Photoreceptor
Apoptosis
Retina
Degeneration
