摘要
目的了解在哺乳动物整体试验中自来水有机浓集物对机体的损害作用。方法用ＸＡＤ２树脂吸附自来水中有机污染物，采用灌胃或腹腔注射的途径对小鼠进行染毒，测定了骨髓嗜多染红细胞微核率、睾丸染色体畸变率，免疫功能指标及肝脏Ｓ９组分的乙氧基异吩恶唑Ｏ脱乙基酶（ＥＲＯＤ）活性。结果在高剂量（相当２００Ｌ／ｋｇ自来水，３天）染毒条件下，微核率和睾丸染色体畸变率分别增加１．７倍和３．５倍；而在低剂量（相当６Ｌ／ｋｇ自来水，１４天）条件下，机体的体液免疫和非特异性免疫功能明显受到抑制；此外自来水有机浓集物还可明显诱导ＥＲＯＤ活性，且有良好的剂量依赖关系。结论自来水有机浓集物可引起小鼠体细胞及生殖细胞的染色体损伤，免疫功能下降，并可诱导肝脏微粒体细胞色素Ｐ４４８活性，提示自来水有机浓集物对哺乳动物可能具有一定的潜在致癌危险性。
Objective To study the harmful effects of organic concentrates from tap water on mammal animals in vivo. Methods Organic pollutants were absorbed with resin XAD 2 from tap water and administered the mice via a stomach tube or intraperitoneal injection. Micronucleus rate in bone marrow polychromatic erythrocytes, testicular chromosome aberration rate, indices for immune functions and the activity of ethoxyresourufin o deethylase (EROD)in S 9 fraction of the liver were determined. Results Micronucleus rate and chromosome aberration rate increased by 1.7 and 3.5 folds, respectively, after administration of high dose of organic pollutants from the tap water(200 liters of tap water per kilograms of body weight)for three days. Humoral immunity and non specific immune functions were obviously inhibited after administration of low dose (6 L/kg). In addition, organic concentrates from tap water also could significantly induce the activity of EROD, with a good dose dependent relationship. Conclusion Organic concentrates from tap water could cause chromosome damage to somatic and germ cells and decrease in immune functions in the mice, as well as they could induce the activity of cytochrome P448 in the liver microsome. It suggested that organic pollutants concentrated from tap water had potential carcinogenic effects on mammal animals.
出处
《中华预防医学杂志》
CAS
CSCD
北大核心
1999年第3期149-151,共3页
Chinese Journal of Preventive Medicine
关键词
水污染
自来水
有机污染物
危害
实验研究
Hazardous substances Water supply Chromosome aberrations Immunity Oxidoreductases Cytochrome P 450
