摘要
背景:骨形态发生蛋白2和血管内皮生长因子可以促进新骨的形成,提高骨不连的治愈率。目的:观察大鼠股骨骨不连修复过程中骨缺损区骨形态发生蛋白2与血管内皮生长因子mRNA的表达情况。方法:SD大鼠股骨左建立骨不连模型后,随机分为7组,分别于造模后1,3,7,14,21,28,35d,处死取材。大鼠右股骨不做缺损直接缝合伤口,作为对照。RT-PCR技术检测大鼠股骨骨形态发生蛋白2和血管内皮生长因子mRNA的表达。结果与结论:大鼠股骨骨不连损伤后,损伤周围区骨形态发生蛋白2与血管内皮生长因子mRNA表达上升,至7d时达到顶峰,而后随时间的延长而下降;而在损伤中心区骨形态发生蛋白2与血管内皮生长因子mRNA表达延迟,且表达量低于损伤周围区。提示大鼠股骨骨不连缺损中心区域在修复早期骨形态发生蛋白2与血管内皮生长因子mRNA的低表达及时间滞后可能是其骨不连发生的重要原因,在相应的时期补充外源性骨形态发生蛋白2与血管内皮生长因子可能助于骨不连的修复愈合。
BACKGROUND：Bone morphogenetic protein-2（BMP-2） and vascular endothelial growth factor（VEGF） can promote new born formation,and elevate cure rate of bone nonunion.OBJECTIVE：To observe the dynamic mRNA expression of BMP-2 and VEGF during restoration of rat femoral nonunion.METHODS：SD rats were established femoral nonunion models and randomly divided into 7 groups,rats were sacrificed at 1,3,7,14,21,28 and 35 days after model preparation.Wounds of rats in the control group were directly sutured without defects.The mRNA expression of BMP-2 and VEGF were detected using RT-PCT technique.RESULTS AND CONCLUSION：The mRNA expression of BMP-2 and VEGF at the outer zone of injury were increased after injury,reached a peak at 7 days,and the decreased with time prolonged.However,the mRNA expression of BMP-2 and VEGF at the central zone of injury were delayed expressed and smaller than that of the outer zone.Low and delay expression of BMP-2 and VEGF in the central zone during the early stage may be an important reason for bone nonunion,thus,supplying extrinsic source of BMP-2 and VEGF in early stage may be contribute to the repairing of nonunion.
出处
《中国组织工程研究与临床康复》
CAS
CSCD
北大核心
2010年第37期6857-6860,共4页
Journal of Clinical Rehabilitative Tissue Engineering Research
基金
镇江市社会发展基金(SH2002019)
课题名称:骨髓间充质干细胞体外扩增回植对骨缺损的作用~~
