摘要
背景:在众多的血管生长因子中,血管内皮生长因子是最有力的血管生成因子,它具有促进血管内皮细胞分裂、增殖,细胞质钙化聚集并能诱导血管生成等作用,且对于成骨细胞、破骨细胞的增殖、分化及功能活性具有重要调节作用。目的:观察雌激素对成骨细胞上血管内皮生长因子表达的影响。方法:取新生48h大鼠颅盖骨,酶解消化得到成骨细胞,并进行体外培养。采用RT-PCR法测定10-10,10-8,10-6,10-4mol/L17β-雌二醇作用成骨细胞后血管内皮生长因子mRNA的表达。结果与结论:RT-PCR检测结果显示,血管内皮生长因子mRNA在原代成骨细胞内稳定表达;半定量分析证实,不同浓度17β-雌二醇组间血管内皮生长因子mRNA的表达量呈现一定规律,随着17β-雌二醇浓度的升高,血管内皮生长因子mRNA的表达量逐渐增加,10-6mol/L左右时,血管内皮生长因子mRNA的表达量最高,超过此剂量,血管内皮生长因子的表达下降。提示雌激素促进成骨细胞上血管内皮生长因子的表达上调,并存在剂量依赖性。
BACKGROUND：Vascular endothelial growth factor（VEGF） is a major angiogenesis factor,characterizing by promoting cell division,proliferation,cytoplasm calcification and aggregation,as well as inducing angiogenesis;on the other hand,it also adjusts proliferation,differentiation,and functional activity of osteoblast and osteoclast.OBJECTIVE：To investigate the effect of estrogen on VEGF expression in rat osteoblasts.METHODS：Calvaria osteoblasts derived from 48-hour-old rats were cultured to obtain osteoblast.RT-PCR was employed to detect the VEGF mRNA expression following induction of 17β-estradiol at concentrations of 10-10,10-8,10-6,and 10-4 mol/L.RESULTS AND CONCLUSION：RT-PCR demonstrated that VEGF mRNA was stably expressed in primary cultured osteoblast.Semi-quantitative analysis demonstrated that VEGF mRNA expression was in a certain law following 17β-estradiol induction at varying concentrations,i.e.,with the increasing concentration of 17β-estradiol,VEGF mRNA expression was increased.At 10-6 mol/L,the VEGF mRNA expression peaked,and when 17β-estradiol was over this dose,the expression was gradually decreased.This suggested that estrogen promoted VEGF mRNA expression,showing a dose-dependent manner.
出处
《中国组织工程研究与临床康复》
CAS
CSCD
北大核心
2010年第24期4390-4392,共3页
Journal of Clinical Rehabilitative Tissue Engineering Research
