摘要
背景:有报道指出,骨髓间充质样干细胞和骨髓单个核细胞在多种疾病中被证明有疗效,骨髓间充质样干细胞在肺纤维化模型中也有应用。目的:比较骨髓间充质样干细胞和骨髓单个核细胞对博莱霉素A5所致大鼠肺泡炎和早期纤维化的治疗作用。方法:收集Wistar雄性幼鼠的骨髓间充质样干细胞、骨髓单个核细胞并分别进行DAPI标记。21只Wistar雌性大鼠气管内注入博莱霉素A5制作肺损伤模型,随机均分为骨髓间充质样干细胞治疗组、骨髓单个核细胞治疗组和模型组。造模后第7天处死大鼠,取肺组织病理切片观察炎症和纤维化情况;荧光显微镜下检测DAPI标记细胞;ELISA法检测肺组织匀浆羟脯氨酸和肿瘤坏死因子的含量。结果与结论:①在骨髓间充质样干细胞治疗组、骨髓单个核细胞治疗组肺组织冰冻切片中均见到DAPI标记的外源细胞。②模型组肺泡炎最严重,骨髓间充质样干细胞治疗组肺泡炎最轻,各组比较,差异有显著性意义(P<0.05)。③模型组肺组织匀浆中羟脯氨酸和肿瘤坏死因子α含量最多,骨髓间充质样干细胞治疗最少,各组比较差异有显著性意义(P<0.05)。提示骨髓间充质样干细胞和骨髓单个核细胞均可定植于损伤肺组织,并能有效阻止博莱霉素A5诱导的大鼠肺泡炎和早期纤维化,前者作用更为显著。
BACKGROUND：Previous studies demonstrated that many diseases can be effectively treated by using both bone marrow-derived mesenchymal-like stem cells （BM-MSCs） and bone marrow-derived mononuclear cells （BM-MNCs）.BM-MSCs also have been used in lung fibrosis models.OBJECⅡVE：To compare the curative effect of BM-MSCs and BM-MNCs for treating bleomycin A5-induced alveolitis and early fibrosis.METHODS：BM-MSCs and BM-MNCs from Wistar male young rats were cultured and marked with 4, 6-diamidino-2-phenylindole （DAPI）.A total of 21 female Wistar rats were intratracheally injected with bleomycin A5 to establish pulmonary fibrosis models.The rats were randomly divided into BM-MSCs group, BM-MNCs group and model group.The rats were sacrificed on day 7 following model induction.Pathological sections of pulmonary tissues were obtained to observe inflammation and fibrosis.Fluorocyte marked with DAPI was detected by fluorescence microscopy.The levels of hydroxyproline and tumor necrosis factors-α （TNF-α） were measured by enzyme linked immunosorbent assay （ELISA）.RESULTS AND CONCLUSION：① In BM-MSCs group and BM-MNCs group, BM-MSCs and BM-MNCs marked with DAPI were detected in lung frozen section.②Pulmonary alveolitis was most serious in model group, pulmonary alveolitis and fibrosis in BM-MNCs group was more serious than BM-MSCs group, and significant differences were detected among groups （P〈0.05）.③ Hydroxyproline and TNF-α levels were highest in model group, and lowest in the BM-MSCs group, and significant differences were detected among groups （P〈0.05）.Results suggest that both BM-MSCs and BM-MNCs can be planted in the impaired lung tissue, and effectively prevent the development of bleomycin-induced pulmonary alveolitis and early fibrosis.The curative effect of BM-MSCs is better than that of BM-MNCs.
出处
《中国组织工程研究与临床康复》
CAS
CSCD
北大核心
2010年第36期6672-6676,共5页
Journal of Clinical Rehabilitative Tissue Engineering Research
基金
广州市医药卫生科技项目(编号:2008-ZDi-04),项目名称:Smad7和HGF基因在矽肺纤维化的作用及靶向治疗的研究~~
