摘要
背景:内皮祖细胞在维持内皮系统功能及血管损伤后的修复中起重要作用,已广泛应用到心血管、下肢缺血、血管修复等多种疾病,但在炎症疾病及肺损伤中的研究较少。目的:观察内皮祖细胞移植对急性肺损伤/急性呼吸窘迫综合征肿瘤坏死因子α及白细胞介素10的影响,探讨细胞移植能否改善急性肺损伤/急性呼吸窘迫综合征的炎症状态。方法:同遗传背景SD大鼠30只随机均分为正常对照组、肺损伤组、细胞移植组。采用密度梯度离心法分离、培养SD大鼠的骨髓内皮祖细胞。肺损伤组、细胞移植组大鼠经尾静脉注射脂多糖建立急性肺损伤模型;正常对照组仅给予等量的磷酸盐缓冲溶液。造模半小时后,正常对照组、细胞移植组大鼠经尾静脉注入内皮祖细胞悬液;肺损伤组大鼠同法注入等量的磷酸盐缓冲溶液。结果与结论:与肺损伤组相比,细胞移植组中白细胞介素10的水平显著增加(P<0.001),肿瘤坏死因子α的表达下调,但差异无显著性意义(P>0.05)。细胞移植促进白细胞介素10的表达,下调肿瘤坏死因子α的表达,能改善损伤肺组织的炎症状态。
BACKGROUND： Endothelial progenitor cells play an important role in maintaining normal endothelial function as well as endothelial repairing after vascular injury, which has been widely applied to multiple clinical disorders including cardiovascular disease, limb ischemia and vascular repair. But the application studies of endothelial progenitor cells are much fewer in inflammatory diseases and lung injury. OBJECTIVE： To observe the effect of endothelial progenitor cell transplantation on expressions of tumor necrosis factor-α and interleukin-10 in acute lung injury/acute respiratory distress syndrome rats and to explore whether cell transplantation can improve the inflammatory state of acute lung injury/acute respiratory distress syndrome. METHODS： Thirty Sprague Dawley rats of the same genetic background were randomly divided into three groups with 10 rats in each group： normal control group, lung injury group and cell transplantation group. The endothelial progenitor cells were isolated and cultured with density gradient centrifugation method. Rats in the lung injury group and cell transplantation group were injected with lipopolysaccharide through tail vein to make the acute lung injury models. Rats in normal control group only received phosphate buffered solution with the same dose. Half an hour after modeling, rats in the normal control group and cell transplantation group were injected with bone marrow-derived endothelial progenitor cells suspension through tail vein, and rats in lung injury group received the same amounts of phosphate buffered solution. RESULTS AND CONCLUSION： Compared with the lung injury group, the expression of interleukin-10 was significantly increased in the cell transplantation group （P＆lt;0.001）, but the expression of tumour necrosis factor-α was reduced, and the difference was not significant （P＆gt;0.05）. It indicated that transplantation of endothelial progenitor cells could up-regulate interleukin-10 expression and down-regulate tumour necrosis factor-α expression, and could significantly improve the inflammatory state in injury lung tissues.
出处
《中国组织工程研究》
CAS
CSCD
2013年第10期1780-1784,共5页
Chinese Journal of Tissue Engineering Research
基金
湖北省自然科学基金(2011CDB024)~~
关键词
干细胞
干细胞移植
内皮祖细胞
细胞移植
肿瘤坏死因子α
白细胞介素10
肺组织
炎症
省级基金
干细胞图片文章
stem cells
stem cell transplantation
endothelial progenitor cells
tumour necrosis factor-α
interleukin-10
lung tissue
inflammation
other grants-supported paper
stem cell photographs-containing paper
