摘要
为探讨植物转基因技术在桑树品质改良上的应用途径，构建了含大豆球蛋白亚基（ＡｌａＢｌｂ）基因的表达载体，将其转入根癌农杆菌后，用农杆菌介导法转化桑树叶盘与茎尖，经卡那霉素抗性筛选培养，再生形成了不定芽及试管幼苗，ＰＣＲ检测证实ＡｌａＢｌｂ亚基基因已转入桑树。
In order to investigate the possibility of improving mulberry quality by transgenic techniques,expression vector carrying glycinin subunit AlaBlb gene was constructed and transferred into Agrobacterium tumefaciens. And then,leaf disk and stem apex of mulberry were used as receptor materials of genetic transformation induced by Agrobacterium. The regeneration plantlets with kanamycin resistance were obtained from these materials.Results by PCR indicated that AlaBlb subunit gene was transformed into mulberry plant.
出处
《蚕业科学》
CAS
CSCD
1999年第1期5-10,共6页
ACTA SERICOLOGICA SINICA
基金
国家自然科学基金
关键词
桑树
转基因
大豆球蛋白
根癌农杆菌
Mulberry Transformation Glycinin Agrobacterium tumefacien
