摘要
在克隆水稻谷蛋白基因 Gt1启动子的基础上 ,以农杆菌双元载体 p CAMBIA130 1为起点 ,构建出以该启动子带动大豆球蛋白 A1a B1b亚基基因表达的双元载体 ,并用农杆菌介导法转化水稻获得转基因植株 .经 PCR检测和后代分析 ,大豆球蛋白基因已整合入水稻基因组 。
Based on the previous work of cloning of upstream sequence of a rice glutelin gene Gt1, an Agrobacterium binary vector based on pCAMBIA1301 was reconstructed with a soybean glycinin gene directed by the tissue specific promoter, and was used in rice transfomation. The target gene was successfully introduced into calli of an early japonica rice variety with Agrobacterium mediated transformation and several transgenic plants were obtained. PCR analysis of the transformed plants and Hyg resistance tests of T 0 seeds showed the integration of the target gene and its stable inheritance to offsprings.
出处
《浙江大学学报（农业与生命科学版）》
CAS
CSCD
北大核心
2001年第5期495-499,共5页
Journal of Zhejiang University:Agriculture and Life Sciences
基金
国家 8 6 3计划 (10 1- 0 1- 0 1- 0 1- 3)
浙江省8812计划
关键词
水稻
农杆菌转化
启动子
贮藏蛋白
大豆球蛋白基因
品质
Oryza sativa
Agrobacterium mediated transformation
promoter
storage protein
Glycinin gene (Gly1)
