摘要
利用倒置显微镜和透射电子显微镜技术观察印楝素A诱导草地贪夜蛾Spodoptera frugperda蛹卵巢Sf9细胞凋亡的显微和超微形态变化.结果表明：1.5μg.mL-1印楝素A对Sf9细胞具有显著的凋亡诱导作用,从凋亡小体和贴壁细胞数量变化判断,印楝素A处理12 h,细胞开始出现少量凋亡小体,12~24 h凋亡小体逐渐增多,但贴壁细胞未见明显减少,因此24 h之前（0~24 h）属于凋亡早期阶段;24~36 h凋亡小体大量出现,贴壁细胞逐渐减少,属于凋亡中期;处理36 h以上,凋亡小体和贴壁细胞急剧减少,细胞凋亡进入晚期.观察诱导24 h的细胞超微结构发现,胞内的主要细胞器在数量或形态上发生了显著变化,包括溶酶体数量显著增加,线粒体内部嵴变模糊,内质网从囊泡状变成线管状,细胞核出现分页、皱缩以及区域性膨大等.
The morphological changes of microstructure and ultra-structure of apoptotic Sf9 cells isolated from ovary of pupa of Spodoptera frugperda,induced by azadirachtin A were studied by using the techniques of transmission electron microscope and inverted phase microscope.The results showed that the significant apoptosis was induced by azadirachtin A at a mass concentration of 1.5 μg·mL-1.By identifying the number of apoptotic bodies and adherent cells,the early apoptotic stage of Sf9 cells appeared within 24 h due to appearance and increasing of apoptotic bodies,but the number of adherent cells was not decreased obviously after treated by azadirachtin A for 12-24 h;While the apoptotic bodies increased and adherent cells decreased regularly after treated for 24-36 h,which indicated that this period was the apoptosis mid stage;and then the late stage of the apoptosis after treated for more than 36 h because of sharply decrease of adherent cells and apoptotic bodies.The main organelles changing significantly in morphology or number were found after observing the ultra-structure of Sf9 cells induced for 24 h,including markedly increase of lysosomes,endoplasmic reticulum changing from vesicles to line pipe-like,nuclear pagination,crimpled nuclear,partly swollen of nuclear membrane.
出处
《华南农业大学学报》
CAS
CSCD
北大核心
2010年第4期52-58,共7页
Journal of South China Agricultural University
基金
国家自然科学基金(30871660)
教育部全国优秀博士学位论文作者专项基金(2004061)
