摘要
目的 观察大鼠肝硬化门脉高压形成过程中外周血浆内皮素-1(ET-1)和一氧化氮(NO)的动态变化,以探讨两种物质在门脉高压高动力循环中的作用。方法 肝硬化门脉高压组大鼠模型用四氯化碳加乙醇制备,对照组只注射等量橄榄油。放免法检测血浆ET-1水平,硝酸还原酶法检测NO,观察两者在不同时期的变化。结果 模型制备过程中的第0、2和6周未见两组动物之间存在NO和ET-1水平的显著性差异,第10周时有ET-1水平的降低和NO水平的显著升高,同时有门脉压力的增高和体循环平均动脉压的降低。结论ET-1水平的降低和NO水平的增高是引起肝硬化门脉高压高动力循环产生的重要原因。
Objective To observe the dynamic changes of endothelin-1 (ET-1) and nitric oxide (NO) in plasma of the rats during the process of cirrhotic hypertension. Methods Carbon tetrachloride plus ethanol were used for the induction of cirrhotic hypertension. The concentration of ET-1 was detected using radioimmunoassay and the level of NO was determined by the method of nitric acid reductase. Results In the 0,2 and 6 weeks during the formation of cirrhosis, there existed no significant difference between the model group and the control group. But at the tenth week, the level of ET-1 decreased and the concentration of NO elevated significantly. At the same time,the portal pressure raised and the mean arterial pressure lowered. Conclusion The reduction of ET-1 and the elevation of NO contribute markedly to the cirrhotic hyperdynamic circulatory status.
出处
《肝脏》
1999年第2期84-86,共3页
Chinese Hepatology
关键词
肝硬化
门静脉高压
内皮素-1
一氧化氮
Hepatic cirrhosis Portal hypertension Hyperdynamic circulation Endothelin-1 Nitric oxide
