摘要
目的建立HPLC法同时测定复方环吡酮胺溶液中苯佐卡因和环吡酮胺两组分的含量。方法采用美国Agilent ZORBAX SB-C18色谱柱,以甲醇-乙腈-水-冰醋酸(15∶35∶50∶0.3)为流动相。检测波长为300nm,流速为1mL.min-1。结果苯佐卡因和环吡酮胺在15min内完全分离,两者在10～120mg.L-1范围内线性关系良好,平均回收率分别为101.15%和100.70%,相对标准偏差(RSD)均小于2%(n=9);日内精密度和日间精密度(RSD)均小于2%(n=5)。结论本法准确、可靠、重复性好,可有效控制环吡酮胺溶液的质量。
Objective To establish a method for simultaneous determination of benzocain and cidopirox olamine in Compound Cidopirox Olamine Solution.Methods The sample was separated on Agilent ZORBAX SB-C18 column,with the mobile phase consisting of methanol-acetonitrile-water-acetic acid（15∶35∶50∶0.3）,the flow rate of 1.0mL·min-1,and the detection wavelength at 300nm.Results Within the range of 10～120μg·mL-1 of benzocain and cidopirox olamine,the calibration curve was linear.The mean recovery rate（%） of benzocain was 101.15（RSD=1.53%,n=9）,and the mean recovery rate（%） of cidopirox olamine was 100.70（RSD=1.64%,n=9）.Conclusion The method is accurate,reliable,stable and reproducible.It can be used for determination of Compound Cidopirox Olamine Solution
出处
《中国药事》
CAS
2010年第10期997-999,共3页
Chinese Pharmaceutical Affairs
