摘要
制备环吡酮胺发用洗剂,并采用HPLC法测定环吡酮胺含量。采用WatersXTerraRMSC18(3.0mm×100mmcolumn)色谱柱,以0.096%的乙二胺四乙酸二钠溶液-冰醋酸(750:0.1)为流动相A,乙腈为流动相B,采用梯度洗脱,流速0.7mL/min,检测波长为304nm,柱温:30℃,进样量:20μL。结果表明,环吡酮胺在0.06~0.09mg/mL范围内,浓度与峰面积的线性关系良好(r=0.9938),回收率、精密度符合要求,空白辅料对环吡酮胺的测定没有干扰,含量测定方法简便、灵敏、准确。
Preparation of ciclopirox olamine hair lotion,determination of ciclopirox olamine content by HPLC.A Waters X TerraR MS C18(3.0 mm×100 mm column)chromatographic column was used,0.096%disodium ethylenediaminetetraacetate solution-glacial acetic acid(750:0.1)was used as mobile phase A,acetonitrile was used as mobile phase B,and gradient elution was used.The flow rate was 0.7 mL/min,the detection wavelength was 304 nm,the column temperature:30°C,and the injection volume:20μL.The results showed that ciclopirox olamine was in the range of 0.06~0.09 mg/mL,and the linear relationship between the concentration and the peak area was good(r=0.9938),and the recovery and precision meet the requirements.Blank excipients did not interfere with the determination of ciclopirox olamine,and the content determination method is simple,sensitive and accurate.
作者
石锦峰
李媛媛
徐旭辉
张帅
董自波
Shi Jinfeng;Li Yuanyuan;Xu Xuhui;Zhang Shuai;Dong Zibo(School of Pharmacy,Jiangsu Ocean University,Jiangsu,222005;Excellent Science and Technology Innovation Team of Jiangsu Provincial Department of Education,Jiangsu Marine Pharmaceutical Resources Development Engineering Research Center,Jiangsu,222005)
出处
《当代化工研究》
CAS
2023年第12期39-41,共3页
Modern Chemical Research
关键词
环吡酮胺
HPLC
制备
含量测定
ciclopirox olamine
HPLC
preparation
content determination
