摘要
目的：采用大鼠全脑缺血再灌注模型，观察海马组织兴奋性氨基酸、线粒体钙、钙调素含量的变化，研究分析脑缺血再灌注损伤中兴奋性氨基酸与钙平衡紊乱的变化和作用。方法：测定假手术组，缺血３０ｍｉｎ再灌注６０ｍｉｎ和缺血３０ｍｉｎ再灌注１２ｈ组，脑海马组织兴奋性氨基酸、线粒体钙、钙调素的含量。结果：缺血３０ｍｉｎ再灌注６０ｍｉｎ海马组织兴奋性氨基酸明显低于假手术组（Ｐ＜０．０１），线粒体钙、钙调素含量显著性高于假手术组（Ｐ＜０．０１），缺血３０ｍｉｎ再灌注１２ｈ组同假手术组比较没有显著性差异（Ｐ＞０．０５）。结论：我们从鼠脑缺血再灌注时线粒体钙。
Objective
:In order to study the relation between excitatory amino acid (EAA),calcium abnormal balance
and cerebral ischemic reperfusion damage,the changes of rat hippocampus EAA,mitochondria
calcium (MCa),calmodulin (CaM) were observed on cerebral ischemic reperfusion model (4VO).
Methods :We measured respectively hippocampus glutamate (Glu),aspartate (Asp),MCa,CaM
contents on sham operated group,ischemia 30 min reperfusion 60 min and ischemia 30 min
reperfusion 12h group. Results :We found that the EAA contents significantly decreased and the
MCa,CaM contents significantly increased in ischemia 30 min reperfusion 60 min group,the
EAA,MCa,CaM contents nearly came back to control's,the change of EAA contents had
cooperative relation to that of MCa,CaM contents during ischemic reperfusion damage.
Conclusion :According to changes of EAA,MCa,CaM,we suggested that calcium abnormal
balance play a important role in ischemic reperfusion cerebral damage produced by EAA.
出处
《卒中与神经疾病》
1999年第2期92-93,共2页
Stroke and Nervous Diseases
关键词
脑缺血
再灌注损伤
兴奋性氨基酸
线粒体钙
Cerebral ischemic reperfusion damageExcitatory amino acidMitochondria
calciumCalmodulin
