摘要
目的:观察阳极氧化处理对近β钛合金Ti-5Zr-3Sn-5Mo-15Nb(TLM)表面处理后表面特性的变化及其对成骨细胞早期附着的影响。方法:用稳压直流电源在5V和20V2个不同电压下、在0.5%氢氟酸中对TLM表面进行阳极氧化处理,另设一抛光组为对照样品于空气中450℃退火1h。用场发射扫描电镜观察试样表面形貌;X射线衍射仪(XRD)分析试样表面晶相;测量水和二碘甲烷2种液体在试样表面的接触角并计算表面能;细胞免疫荧光染色,显微镜下计数评价成骨细胞早期附着。结果:TLM经20V阳极氧化处理后,形成管径较大(80～100nm)、管分布较稀疏的纳米管形貌;经5V阳极氧化处理形成孔径较小(8～12nm)、孔分布较密集的纳米孔形貌。XRD分析表明TLM表面氧化钛纳米管经退火后转变为锐钛矿。TLM经阳极氧化处理后表面能明显增大,且纳米管的表面能略大于纳米孔。成骨细胞在纳米管表面的早期附着数量明显高于纳米孔和抛光表面。结论:阳极氧化处理可在TLM表面形成纳米多孔形貌,且该形貌受阳极氧化电压影响。成骨细胞在20V阳极氧化所形成的纳米管表面的早期附着优于其它2组,这可能与其特殊的表面形貌及较高的表面能有关。
Objective：To evaluate the influence of anodization of near β titanium alloy Ti-5Zr-3Sn-5Mo-15Nb（TLM）on initial osteoblasts adhesion.Methods：TLM specimens were anodized using a DC power supply at two different voltages 5 V and 20 V,and the morphology of the specimens was observed using field emission scanning electron microscope.The crystallinity of the specimens was evaluated by X-ray diffraction（XRD）.Contact angle measurements were carried out with two liquids namely ultrapure water and diiodomethane,then the surface energy was calculated.Cell adhesion was evaluated by counting the stained cells under fluorescence microscope.Results：Anodization of TLM formed nanostructured topographies.A texture with sparsely distributed nanotubes of 80-100 nm in diameter was grown on TLM after 20 V anodization,and a nanoporus surface with pore size of 8-12 nm in diameter was formed at 5 V.XRD spectra showed that the annealed oxide layer formed by anodization were in anatase phase.The surface energy of the nanotubular surface was a little higher than that of the nanoporous surface,obviously higher than that of polished surfaces.Initial osteoblast adhesion was strikingly higher on the nanotubular surfaces than that on the nanoporous and polished surfaces.Conclusion：TLM nanotubular surfaces anodized at high voltage have great surface energy and large tube diameter,show enhanced osteoblasts initial adhesion ability.
出处
《实用口腔医学杂志》
CAS
CSCD
北大核心
2010年第5期597-601,共5页
Journal of Practical Stomatology
