摘要
目的:建立灵芝颗粒的质量标准。方法:采用薄层色谱(TLC)法对方中灵芝、柴胡进行定性鉴别;采用高效液相色谱法测定五味子中五味子甲素、五味子乙素含量。结果:TLC斑点清晰,分离度好,阴性对照无干扰;五味子甲素进样量、五味子乙素进样量分别在0.060～0.60、0.082～0.82μg范围内与各自峰面积积分值呈良好线性关系(r分别为0.9996、0.9995);平均回收率分别为99.02%、99.06%,RSD分别为0.45%(n=6)、0.48%(n=6)。结论:定性定量方法快速、准确、专属性强,所建标准可用于灵芝颗粒的质量控制。
OBJECTIVE：To establish the quality standard of Lingzhi granules.METHODS：The qualitative identification of Ganoderma and Bupleuri Radix was carried out by TLC.The content of deoxyschizandrin and γ-Schisandrin in Schisandra chinensis was determined by HPLC.RESULTS：The TLC sports were fairy clear and separated well without interference of negative sample.The linear ranges were 0.060～0.60 μg for deoxyschizandrin（r=0.999 6）,0.082～0.82 μg for γ-Schisandrin（r=0.999 5）,respectively.The average recoveries were 99.02%（RSD=0.45%,n=6） and 99.06%（RSD=0.48%,n=6）.CONCLUSION：The method is rapid,accurate and specific.It can be used for the quality control of Lingzhi granules.
出处
《中国药房》
CAS
CSCD
北大核心
2010年第39期3722-3724,共3页
China Pharmacy
