摘要
目的:建立以高效液相色谱法测定麦冬药材中麦冬皂苷Ra和慈溪麦冬皂苷A含量的方法。方法:色谱柱为Waters C18(150mm×4.6mm,5μm),流动相为乙腈-水(35∶65),流速为1.0mL·min-1,检测波长为208nm,柱温为室温。结果:麦冬皂苷Ra、慈溪麦冬皂苷A的进样量分别在1.88～9.40μg(r=0.9996)、1.96～9.80μg(r=0.9995)范围内与各自峰面积积分值呈良好线性关系;平均回收率分别为100.2%、99.7%,RSD均为1.7%(n=6)。结论:本方法简便、快速、准确,可用于麦冬药材的质量控制。
OBJECTIVE：To determine the contents of ophiopogon Ra and cixi-ophiopogon A from Ophiopogon japonicus by HPLC.METHODS：The determination was performed on Waters C18（150 mm×4.6 mm,5μm） column with mobile phase consisted of acetonitrile-water（35：65） at flow rate of 1.0 mL·min^-1.The detection wavelength was set at 208 nm and the column temperature was room temperature.RESULTS：The linear range of ophiopogon Ra was 1.88～9.40 μg（r=0.999 6） with an average recovery of 100.2%（RSD=1.7%,n=6）.The liner range of cixi-ophiopogon A was 1.96～9.80 μg（r=0.999 5） with an average recovery of 99.7%（RSD=1.7%,n=6）.CONCLUSION：The method is simple,rapid and accurate for the quality control of O.japonicus.
出处
《中国药房》
CAS
CSCD
北大核心
2010年第39期3704-3705,共2页
China Pharmacy
基金
重庆市科技攻关计划项目(CSTC2008AC5029
CSTC2008AB5116)
