摘要
为建立一个新的人单核细胞来源的泡沫细胞模型，将Ｕ９３７细胞与８０ｍｇ／Ｌ氧化型低密度脂蛋白孵育４８ｈ，应用高效液相色谱检测发现细胞内胆固醇和胆固醇酯增加，胆固醇酯含量大于总胆固醇含量的５０％；应用光镜和透射电镜观察发现细胞胞浆内出现大量的红染颗粒或脂质空泡。提示Ｕ９３７细胞与８０ｍｇ／Ｌ氧化型低密度脂蛋白孵育４８ｈ后，成功建立了人类单核细胞源性泡沫细胞。
Aim To establish a macrophage derived foam cell model from human monocytic U937 cell line. Methods U937 cells were incubated with 80mg/L oxidized low density lipoprotein for 48 hours. High performance liquid chromatography (HPLC) was used for qualitative and quantitative analysis of intracellular cholesterol and cholesteryl esters. Both light microscope (using red oil O staining technique)and transmission electron microscope were applied to observe the morphology of treated and control U937 cells. Results Total intracellular cholesterol in oxidized low density lipoprotein treated U937 cells increased markedly with cholesteryl esters higher than cholesterol identified by HPLC assay. There were also many lipid droplets in these cells both under the electronic microscope and light microscope. Conclusion A novel human monocyte derived foam cell model was established by incubating U937 cells with 80 mg/L oxidized low density lipoprotein for 48 hours.
出处
《中国动脉硬化杂志》
CAS
CSCD
1999年第2期152-154,共3页
Chinese Journal of Arteriosclerosis
关键词
U937细胞系
氧化型
低密度脂蛋白
动脉粥样硬化
U937 Cell Line
Lipoprotein, LDL
High Performance Liquid Chromatography
Cholesterol
Cholesteryl Esters
Foam Cell
Atherosclerosis
