摘要
本文用Ｐｉｃｏ-ＴａｇＴＭ（美国ＷＡＴＥＲＳ公司商标名）氨基酸分析法测定了家兔主动脉壁Ｌ-精氨酸含量，样品（动脉环）先用三氯乙酸和水饱合醚抽提，把所得粗提干粉作Ｐｉｃｏ-ＹａｇＴＭ氨基酸分析。样品Ｌ-精氨酸峰的峰面积经９９１纯度分析软件校正后，再与标准Ｌ-精氨酸的峰面积相比较计算出样品中Ｌ-精氨酸的含量。实验共分为三组，统计学处理的各组Ｌ-精氨酸的含量具有一定的参考价值。
In this paper, the method of PicoTagTM amino acid analysis was introduced to measure the level of L-arginine in aortic wall of rabbits. Firstly, the sample (isolated artergal ring of rabbits) was extracted with trichloroacetic acid and water-saturated ether. The crude product was further assayed by PicoTagTM amino acid analysis. The peak area of L-arginine's was corrected by 991 purity analysis disk, the L-arginine levelwas determined by comparing with standrad L-arginine peak area. Three groups of samples was assayed. The statistical L-arginine level of the three group is valuable.
出处
《中国动脉硬化杂志》
CAS
CSCD
1996年第1期72-74,共3页
Chinese Journal of Arteriosclerosis
关键词
L-精氨酸
分析
方法技术
Pico-Tag￣TM amino acid analysis
L-arginine level
Peak area
