摘要
肿瘤坏死因子和一氧化氮与动脉粥样硬化的进程有着密切关系．本实验发现与轻度修饰低密度脂蛋白共同培养２４小时的巨噬细胞肿瘤坏死因子的产生量是与未修饰低密度脂蛋白共同培养的２倍，同时一氧化氮的产生量是后者的５倍。而与氧化低密度脂蛋白共同培养的巨噬细胞，肿瘤坏死因子产生量逐渐减少，减小量与氧化低密友脂蛋白的浓度和作用时间相关，一氧化氮的产生量则未见增加。这可能是氧化低密度脂蛋白损伤了细胞免疫反应能力，从而加速了巨噬细胞泡沫样变性。
A large number of growth factors and cytokines involved in the processes of atherogenesis.A variety of in vitro and in vivo studies gave indicated that tumor necrosis factor (TNF)and nitric oxide (NO)have close relation to atherogenesis. In order to find out the secretion of TNF and NO production by lipopolysaccharide-stimulated macrophages preincubated with minimally modified low density lipoprotein(MM LDL)and oxidized low density lipoprotein(OLDL). The results were that after 48 hours treated, the secretion of TNF induced by MM LDL increased up to 2-fold, and the increase of NO production could be up to 5-fold. However, OLDL sharply inhibited the production of TNF in a concentration-and time-dependant with maximum inhibition(>90%)after 48 h preincubation with OLDL,and NO production did not increase(P>0.05). Concerning with other studies, we suggested that in the different stages of atherogenesis, tbe amount of production of TNF and NO by vessal cells might be different, but more evidents in vivo are needed to find out this mechanism.
出处
《中国动脉硬化杂志》
CAS
CSCD
1994年第2期72-76,共5页
Chinese Journal of Arteriosclerosis
关键词
低密度脂蛋白
肿瘤坏死因子
一氧化氮
免疫反应
Minimally modified low density lipoprotein
Tumor necrosis factor
Nitric oxide
Immune response
