摘要
为研究急性心肌梗死患者外周血Ｔ淋巴细胞表达人白细胞－ＤＲ抗原的变化。将新鲜的全血或经培养的全血与抗体混合，取有核细胞，加入抗ＩｇＧ·异硫氰酸荧光素和抗ＩｇＧ·藻红蛋白，用流式细胞仪技术进行分析。结果发现：（１）ＣＤ３抗原阳性细胞无显著改变，ＣＤ８抗原阳性细胞和人白细胞－ＤＲ抗原阳性细胞显著增高，ＣＤ４抗原阳性细胞和ＣＤ４／ＣＤ８比值显著降低；（２）ＣＤ３抗原和ＤＲ抗原双阳性细胞和ＣＤ４抗原和ＤＲ抗原双阳性细胞显著增加，ＣＤ８抗原和ＤＲ抗原双阳性细胞无显著变化，ＣＤ４抗原和ＤＲ抗原与ＣＤ８抗原和ＤＲ抗原比值增高；（３）在植物凝集素、沸波醇酯和沸波醇酯＋ｉｏｎｏｍｙｃｉｎ等刺激剂存在的情况下，ＣＤ３抗原和ＤＲ抗原表达降低，ＣＤ８抗原和ＤＲ抗原表达与对照组无显著性差异。提示急性心肌梗死患者Ｔ淋巴细胞表达白细胞－ＤＲ抗原异常。
Aim The changes of human leukocyte antigen DR (HLA DR) antigens expression on the peripheral blood T lymphocyte in patients with acute myocardial infarction were studied with flow cytometric analysis. Methods Blood samples or cultured blood samples were mixed with monoclonal antibody. Whole peripheral blood mononuclear cells were obtained from blood sampies. The mononuclear cells were incubated with fluorescein (fluorescein isothiocyanate, FITC;or phycoerythrin,PE) labeled monoclonal antibodies. The samples were analyzed with flow cytometry (FACStar) on the dual labeling. Results ①There are no significant differences between the patients with acute myocardial infarction and normal controls in CD3+ cells. The CD8+ cells and the human leukocyte antigen DR+ cells had significantly increased in patients with acute myocardial infarction. The CD4+ cells and the CD4/CD8 ratio had significantly decreased in patients with acute myocardial infarction; ②In patients with acute myocardial infarction, the percentages of CD3+DR+ cells and CD4+DR+ cells increased significantly , the CD4 DR/CD8 DR ratios were increased. ③Under the induction of phytohemagglutinin, phorbol dibutyrate or phorbol dibutyrate +ionomycin, the competence of human leukocyte antigen DR expression on CD3+ cell in patients with acute myocardial infarction are decreased significantly. The competence of human leukocyte antigen DR expression on CD8+ cell in patients with acute myocardial infarction have no difference from normal control. Conclusion There are abnormal human leukocyte antigen DR expression on T lymphocyte and its subsets in patients with acute myocardial infarction.
出处
《中国动脉硬化杂志》
CAS
CSCD
1999年第2期133-136,共4页
Chinese Journal of Arteriosclerosis
关键词
心肌梗死
急性
T淋巴细胞
白细胞
DR抗原
Myocardial Infarction,Acute
T Lymphocytes
HLA DR Antigens
Flow Cytometry
Phytohemagglutinins
Phorbol 12,13 Dibutyrate
Ionomycin
CD4 CD8 Ratio
