摘要
目的：建立由催化光度法测定超痕量Cu（Ⅱ）的新方法。方法：在弱酸性介质中，超痕量Cu（Ⅱ）对过氧化氢氧化邻苯三酚红的反应有强烈的催化作用，根据催化剂Cu（Ⅱ）用量与该褪色反应速率间的关系．直接测定超痕量Cu（Ⅱ）。结果：此法检测限为4．6×10-11μg／25ml,测定范围为0～0．1μg／ml，测定催化反应的表观活化能为113.04KJ／mol。结论：该方法简便、快速、费用低，可用于人血清中痕量Cu（Ⅱ）的测定。样品分析结果良好。
object : A new method to determine ultra-trace Cu (II) directly by spectrophotometry is reported. Method :[1] tra-trace Cu (II) in weak acid can catalyze the discolouring oxidization of pyrogallol red by hydrogen peroxide. The reaction rate is relatied to the concentration of Cu(II). Results : The detection limit of this method is 4. 6×10-11μg/ml. the linear respondence is 0-0. 1μg /25ml. the surface activatic energy of the catalytic reaction is 113. 04KJ/mol. Conclusion ：This method is characteried by its simpl icity, rapidity and lower expense and may be used to determine ultra-trace Cu (II) in human blood.
出处
《镇江医学院学报》
1999年第2期182-183,共2页
Journal of Zhenjiang Medical College
关键词
催化动力学
分光光度法
测定
铜
Catalytic kinetics
Spectrophotometry
Cu (Ⅱ) determination
